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TitleStructural Insight into the Mechanism of σ32-Mediated Transcription Initiation of Bacterial RNA Polymerase.
Journal, issue, pagesBiomolecules, Vol. 13, Issue 5, Year 2023
Publish dateApr 25, 2023
AuthorsQiang Lu / Taiyu Chen / Jiening Wang / Feng Wang / Wenlong Ye / Lixin Ma / Shan Wu /
PubMed AbstractBacterial RNA polymerases (RNAP) form distinct holoenzymes with different σ factors to initiate diverse gene expression programs. In this study, we report a cryo-EM structure at 2.49 Å of RNA ...Bacterial RNA polymerases (RNAP) form distinct holoenzymes with different σ factors to initiate diverse gene expression programs. In this study, we report a cryo-EM structure at 2.49 Å of RNA polymerase transcription complex containing a temperature-sensitive bacterial σ factor, σ (σ-RPo). The structure of σ-RPo reveals key interactions essential for the assembly of σ-RNAP holoenzyme and for promoter recognition and unwinding by σ. Specifically, a weak interaction between σ and -35/-10 spacer is mediated by T128 and K130 in σ. A histidine in σ, rather than a tryptophan in σ, acts as a wedge to separate the base pair at the upstream junction of the transcription bubble, highlighting the differential promoter-melting capability of different residue combinations. Structure superimposition revealed relatively different orientations between βFTH and σ from other σ-engaged RNAPs and biochemical data suggest that a biased σ-βFTH configuration may be adopted to modulate binding affinity to promoter so as to orchestrate the recognition and regulation of different promoters. Collectively, these unique structural features advance our understanding of the mechanism of transcription initiation mediated by different σ factors.
External linksBiomolecules / PubMed:37238608 / PubMed Central
MethodsEM (single particle)
Resolution2.49 Å
Structure data

34849

8hkc

EMDB-34849, PDB-8hkc:
Cryo-EM structure of E. coli RNAP sigma32 complex
Method: EM (single particle) / Resolution: 2.49 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • escherichia coli k-12 (bacteria)
  • escherichia coli (E. coli)
KeywordsTRANSCRIPTION/DNA / transcription / RNA polymerase / sigma factor / heat shock response / TRANSCRIPTION-DNA complex

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