EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Allosteric modulation of ryanodine receptor RyR1 by nucleotide derivatives.
ジャーナル・号・ページ	Structure, Vol. 31, Issue 7, Page 790-800.e4, Year 2023
掲載日	2023年7月6日
著者	Spencer Cholak / James W Saville / Xing Zhu / Alison M Berezuk / Katharine S Tuttle / Omid Haji-Ghassemi / Francisco J Alvarado / Filip Van Petegem / Sriram Subramaniam /
PubMed 要旨	The coordinated release of Ca from the sarcoplasmic reticulum (SR) is critical for excitation-contraction coupling. This release is facilitated by ryanodine receptors (RyRs) that are embedded in the ...The coordinated release of Ca from the sarcoplasmic reticulum (SR) is critical for excitation-contraction coupling. This release is facilitated by ryanodine receptors (RyRs) that are embedded in the SR membrane. In skeletal muscle, activity of RyR1 is regulated by metabolites such as ATP, which upon binding increase channel open probability (P). To obtain structural insights into the mechanism of RyR1 priming by ATP, we determined several cryo-EM structures of RyR1 bound individually to ATP-γ-S, ADP, AMP, adenosine, adenine, and cAMP. We demonstrate that adenine and adenosine bind RyR1, but AMP is the smallest ATP derivative capable of inducing long-range (>170 Å) structural rearrangements associated with channel activation, establishing a structural basis for key binding site interactions that are the threshold for triggering quaternary structural changes. Our finding that cAMP also induces these structural changes and results in increased channel opening suggests its potential role as an endogenous modulator of RyR1 conductance.
リンク	Structure / PubMed:37192614 / PubMed Central
手法	EM (単粒子)
解像度	2.84 - 3.98 Å
構造データ	40422 8sen EMDB-40422, PDB-8sen: Cryo-EM Structure of RyR1 手法: EM (単粒子) / 解像度: 3.49 Å 40423 8seo EMDB-40423, PDB-8seo: Cryo-EM Structure of RyR1 + ATP-gamma-S 手法: EM (単粒子) / 解像度: 3.92 Å 40424 8sep EMDB-40424, PDB-8sep: Cryo-EM Structure of RyR1 + ADP 手法: EM (単粒子) / 解像度: 3.57 Å 40425 8seq EMDB-40425, PDB-8seq: Cryo-EM Structure of RyR1 + AMP 手法: EM (単粒子) / 解像度: 3.4 Å 40426 8ser EMDB-40426, PDB-8ser: Cryo-EM Structure of RyR1 + Adenosine 手法: EM (単粒子) / 解像度: 3.42 Å 40427 8ses EMDB-40427, PDB-8ses: Cryo-EM Structure of RyR1 + Adenine 手法: EM (単粒子) / 解像度: 3.98 Å 40428 8set EMDB-40428, PDB-8set: Cryo-EM Structure of RyR1 + cAMP 手法: EM (単粒子) / 解像度: 3.42 Å 40429 8seu EMDB-40429, PDB-8seu: Cryo-EM Structure of RyR1 (Local Refinement of TMD) 手法: EM (単粒子) / 解像度: 3.0 Å 40430 8sev EMDB-40430, PDB-8sev: Cryo-EM Structure of RyR1 + ATP-gamma-S (Local Refinement of TMD) 手法: EM (単粒子) / 解像度: 3.17 Å 40431 8sew EMDB-40431, PDB-8sew: Cryo-EM Structure of RyR1 + ADP (Local Refinement of TMD) 手法: EM (単粒子) / 解像度: 2.89 Å 40432 8sex EMDB-40432, PDB-8sex: Cryo-EM Structure of RyR1 + AMP (Local Refinement of TMD) 手法: EM (単粒子) / 解像度: 2.84 Å 40433 8sey EMDB-40433, PDB-8sey: Cryo-EM Structure of RyR1 + Adenosine (Local Refinement of TMD) 手法: EM (単粒子) / 解像度: 2.99 Å 40434 8sez EMDB-40434, PDB-8sez: Cryo-EM Structure of RyR1 + Adenine (Local Refinement of TMD) 手法: EM (単粒子) / 解像度: 3.54 Å 40435 8sf0 EMDB-40435, PDB-8sf0: Cryo-EM Structure of RyR1 + cAMP (Local Refinement of TMD) 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-AMP: ADENOSINE MONOPHOSPHATE / AMP / AMPYM ChemComp-ADN: ADENOSINE / アデノシン ChemComp-ADE: ADENINE / アデニン ChemComp-CMP*: ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE / cAMP
由来	oryctolagus cuniculus (ウサギ) rabbit (ウサギ) homo sapiens (ヒト)
キーワード	TRANSPORT PROTEIN / Calcium ion channel / skeletal muscle / nucleotide / homotetramer