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Structure paper

TitleStructural basis of DNA binding by the WhiB-like transcription factor WhiB3 in Mycobacterium tuberculosis.
Journal, issue, pagesJ. Biol. Chem., Vol. 299, Page 104777-104777, Year 2023
Publish dateMay 19, 2022 (structure data deposition date)
AuthorsWan, T. / Horova, M. / Khetrapal, V. / Li, S. / Jones, C. / Schacht, A. / Sun, X. / Zhang, L.
External linksJ. Biol. Chem. / PubMed:37142222
MethodsX-ray diffraction
Resolution1.35 - 2.44 Å
Structure data

PDB-8cwr:
Complex structure of WhiB3 and the SigmaAr4-RNAP Beta flap tip chimera in space group R3
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-8cwt:
Complex structure of WhiB3 and the SigmaAr4-RNAP Beta flap tip chimera in space group P43212
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-8cyf:
WhiB3 bound to SigmaAr4-RNAP Beta flap tip chimera and DNA
Method: X-RAY DIFFRACTION / Resolution: 2.44 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-SO4:
SULFATE ION

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-NI:
NICKEL (II) ION

ChemComp-HOH:
WATER

ChemComp-CAC:
CACODYLATE ION

Source
  • mycobacterium tuberculosis (bacteria)
  • mycobacterium tuberculosis h37rv (bacteria)
KeywordsTRANSCRIPTION / redox sensor / transcriptional factor / TRANSCRIPTION/DNA/Transferase / protein-DNA complex / TRANSCRIPTION-DNA-Transferase complex

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