+検索条件
-Structure paper
タイトル | NO binds to the distal site of haem in the fully activated soluble guanylate cyclase. |
---|---|
ジャーナル・号・ページ | Nitric Oxide, Vol. 134-135, Page 17-22, Year 2023 |
掲載日 | 2023年5月1日 |
著者 | Rui Liu / Yunlu Kang / Lei Chen / |
PubMed 要旨 | Soluble guanylate cyclase (sGC) is the primary receptor for nitric oxide (NO). The binding of NO to the haem of sGC induces a large conformational change in the enzyme and activates its cyclase ...Soluble guanylate cyclase (sGC) is the primary receptor for nitric oxide (NO). The binding of NO to the haem of sGC induces a large conformational change in the enzyme and activates its cyclase activity. However, whether NO binds to the proximal site or the distal site of haem in the fully activated state remains under debate. Here, we present cryo-EM maps of sGC in the NO-activated state at high resolutions, allowing the observation of the density of NO. These cryo-EM maps show the binding of NO to the distal site of haem in the NO-activated state. |
リンク | Nitric Oxide / PubMed:36972843 |
手法 | EM (単粒子) |
解像度 | 3.1 - 3.2 Å |
構造データ | EMDB-34623, PDB-8hbe: EMDB-34624: Consensus map of human soluble guanylate cyclase in the inactive state at 3.2 angstrom EMDB-34625: Focus refined map of N-lobe of soluble guanylate cyclase in the inactive state at 3.1 angstrom EMDB-34626: Focus refined map of C-lobe of human soluble guanylate cyclase in the inactive state at 3.1 angstrom EMDB-34627, PDB-8hbf: EMDB-34628: Consensus map of human soluble guanylate cyclase in complex with riociguat in the presence of NO at 3.1 angstrom EMDB-34629: Focus refined map of N-lobe of human soluble guanylate cyclase in the NO+Rio state at 3.1 angstrom EMDB-34631: Focus refined map of C-lobe of human soluble guanylate cyclase in the NO+Rio state at 3.1 angstrom EMDB-34632, PDB-8hbh: EMDB-34633: Consensus map of huamn soluble guanylate cyclase in the NO-activated state at 3.2 angstrom EMDB-34634: Focus refined map of N-lobe of human soluble guanylate cyclase in the NO-activated state at 3.1 angstrom EMDB-34635: Focus refined map of C-lobe of human soluble guanylate cyclase in the NO-activated state at 3.1 angstrom |
化合物 | ChemComp-HEM: ChemComp-G2P: ChemComp-MG: ChemComp-GZO: ChemComp-NO: |
由来 |
|
キーワード | SIGNALING PROTEIN / soluble guanylate cyclase |