EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Snapshots of the second-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 1294, Year 2023
掲載日	2023年3月16日
著者	Shanshan Li / Michael Z Palo / Xiaojing Zhang / Grigore Pintilie / Kaiming Zhang /
PubMed 要旨	Group I introns are catalytic RNAs that coordinate two consecutive transesterification reactions for self-splicing. To understand how the group I intron promotes catalysis and coordinates self- ...Group I introns are catalytic RNAs that coordinate two consecutive transesterification reactions for self-splicing. To understand how the group I intron promotes catalysis and coordinates self-splicing reactions, we determine the structures of L-16 Tetrahymena ribozyme in complex with a 5'-splice site analog product and a 3'-splice site analog substrate using cryo-EM. We solve six conformations from a single specimen, corresponding to different splicing intermediates after the first ester-transfer reaction. The structures reveal dynamics during self-splicing, including large conformational changes of the internal guide sequence and the J5/4 junction as well as subtle rearrangements of active-site metals and the hydrogen bond formed between the 2'-OH group of A261 and the N2 group of guanosine substrate. These results help complete a detailed structural and mechanistic view of this paradigmatic group I intron undergoing the second step of self-splicing.
リンク	Nat Commun / PubMed:36928031 / PubMed Central
手法	EM (単粒子)
解像度	2.35 - 3.41 Å
構造データ	33811 7yg8 EMDB-33811, PDB-7yg8: Cryo-EM structure of Tetrahymena ribozyme conformation 5 undergoing the second-step self-splicing 手法: EM (単粒子) / 解像度: 2.97 Å 33812 7yg9 EMDB-33812, PDB-7yg9: Cryo-EM structure of Tetrahymena ribozyme conformation 1 undergoing the second-step self-splicing 手法: EM (単粒子) / 解像度: 2.68 Å 33813 7yga EMDB-33813, PDB-7yga: Cryo-EM structure of Tetrahymena ribozyme conformation 2 undergoing the second-step self-splicing 手法: EM (単粒子) / 解像度: 2.35 Å 33814 7ygb EMDB-33814, PDB-7ygb: Cryo-EM structure of Tetrahymena ribozyme conformation 3 undergoing the second-step self-splicing 手法: EM (単粒子) / 解像度: 2.62 Å 33815 7ygc EMDB-33815, PDB-7ygc: Cryo-EM structure of Tetrahymena ribozyme conformation 4 undergoing the second-step self-splicing 手法: EM (単粒子) / 解像度: 2.65 Å 33816 7ygd EMDB-33816, PDB-7ygd: Cryo-EM structure of Tetrahymena ribozyme conformation 6 undergoing the second-step self-splicing 手法: EM (単粒子) / 解像度: 3.41 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	tetrahymena (真核生物) tetrahymena thermophila (真核生物)
キーワード	RNA / Tetrahymena ribozyme / second step of self-splicing / conformation 5 / conformation 1 / conformation 2 / conformation 3 / conformation 4