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-Structure paper
タイトル | Snapshots of the second-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM. |
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ジャーナル・号・ページ | Nat Commun, Vol. 14, Issue 1, Page 1294, Year 2023 |
掲載日 | 2023年3月16日 |
![]() | Shanshan Li / Michael Z Palo / Xiaojing Zhang / Grigore Pintilie / Kaiming Zhang / ![]() ![]() |
PubMed 要旨 | Group I introns are catalytic RNAs that coordinate two consecutive transesterification reactions for self-splicing. To understand how the group I intron promotes catalysis and coordinates self- ...Group I introns are catalytic RNAs that coordinate two consecutive transesterification reactions for self-splicing. To understand how the group I intron promotes catalysis and coordinates self-splicing reactions, we determine the structures of L-16 Tetrahymena ribozyme in complex with a 5'-splice site analog product and a 3'-splice site analog substrate using cryo-EM. We solve six conformations from a single specimen, corresponding to different splicing intermediates after the first ester-transfer reaction. The structures reveal dynamics during self-splicing, including large conformational changes of the internal guide sequence and the J5/4 junction as well as subtle rearrangements of active-site metals and the hydrogen bond formed between the 2'-OH group of A261 and the N2 group of guanosine substrate. These results help complete a detailed structural and mechanistic view of this paradigmatic group I intron undergoing the second step of self-splicing. |
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手法 | EM (単粒子) |
解像度 | 2.35 - 3.41 Å |
構造データ | EMDB-33811, PDB-7yg8: EMDB-33812, PDB-7yg9: EMDB-33813, PDB-7yga: EMDB-33814, PDB-7ygb: EMDB-33815, PDB-7ygc: EMDB-33816, PDB-7ygd: |
化合物 | ![]() ChemComp-MG: |
由来 |
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![]() | RNA / Tetrahymena ribozyme / second step of self-splicing / conformation 5 / conformation 1 / conformation 2 / conformation 3 / conformation 4 |