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TitleStructural basis of the inhibition of cystathionine gamma-lyase from Toxoplasma gondii by propargylglycine and cysteine.
Journal, issue, pagesProtein Sci., Vol. 32, Page e4619-e4619, Year 2023
Publish dateFeb 22, 2021 (structure data deposition date)
AuthorsFernandez-Rodriguez, C. / Conter, C. / Oyenarte, I. / Favretto, F. / Quintana, I. / Martinez-Chantar, M.L. / Astegno, A. / Martinez-Cruz, L.A.
External linksProtein Sci. / PubMed:36883335
MethodsX-ray diffraction
Resolution1.59 - 2.331 Å
Structure data

PDB-7nl1:
Crystal structure of cystathionine gamma-lyase from Toxoplasma gondii
Method: X-RAY DIFFRACTION / Resolution: 2.331 Å

PDB-8bis:
Crystal structure of cystathionine gamma-lyase from Toxoplasma gondii in complex with DL-propargylglycine
Method: X-RAY DIFFRACTION / Resolution: 2.266 Å

PDB-8biu:
Cystathionine gamma-lyase in complex with cystathionine
Method: X-RAY DIFFRACTION / Resolution: 1.899 Å

PDB-8biv:
Cystathionine gamma-lyase N360S mutant from Toxoplasma gondii
Method: X-RAY DIFFRACTION / Resolution: 2.22 Å

PDB-8biw:
Cystathionine gamma-lyase N360S mutant in complex with DL-propargylglycine
Method: X-RAY DIFFRACTION / Resolution: 1.981 Å

PDB-8bix:
Cystathionine gamma-lyase N360S mutant from Toxoplasma gondii in complex with cystathionine
Method: X-RAY DIFFRACTION / Resolution: 1.59 Å

PDB-8biz:
Cystathionine gamma-lyase from Toxoplasma gondii in complex with cysteine
Method: X-RAY DIFFRACTION / Resolution: 1.891 Å

Chemicals

ChemComp-PLP:
PYRIDOXAL-5'-PHOSPHATE

ChemComp-HOH:
WATER

ChemComp-2AG:
(2S)-2-aminopent-4-enoic acid / inhibitor*YM

ChemComp-2KT:
2-KETOBUTYRIC ACID

ChemComp-GOL:
GLYCEROL

ChemComp-HCS:
2-AMINO-4-MERCAPTO-BUTYRIC ACID

ChemComp-CYS:
CYSTEINE

Source
  • toxoplasma gondii (strain atcc 50611 / me49) (eukaryote)
  • toxoplasma gondii (eukaryote)
KeywordsCYTOSOLIC PROTEIN / transsulfuration pathway / cysteine / hydrogen sulfide / PLP dependent / propargylglgycine / cystathionine gamma-lyase / cystathionine / ketobutyrate / toxoplasma gondii / N360S mutant / propargylglycine / toxoplasma

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