EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural analysis of receptor engagement and antigenic drift within the BA.2 spike protein.
ジャーナル・号・ページ	Cell Rep, Vol. 42, Issue 1, Page 111964, Year 2023
掲載日	2023年1月31日
著者	James W Saville / Dhiraj Mannar / Xing Zhu / Alison M Berezuk / Spencer Cholak / Katharine S Tuttle / Faezeh Vahdatihassani / Sriram Subramaniam /
PubMed 要旨	The BA.2 sub-lineage of the Omicron (B.1.1.529) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant rapidly supplanted the original BA.1 sub-lineage in early 2022. Both lineages ...The BA.2 sub-lineage of the Omicron (B.1.1.529) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant rapidly supplanted the original BA.1 sub-lineage in early 2022. Both lineages threatened the efficacy of vaccine-elicited antibodies and acquired increased binding to several mammalian ACE2 receptors. Cryoelectron microscopy (cryo-EM) analysis of the BA.2 spike (S) glycoprotein in complex with mouse ACE2 (mACE2) identifies BA.1- and BA.2-mutated residues Q493R, N501Y, and Y505H as complementing non-conserved residues between human and mouse ACE2, rationalizing the enhanced S protein-mACE2 interaction for Omicron variants. Cryo-EM structures of the BA.2 S-human ACE2 complex and of the extensively mutated BA.2 amino-terminal domain (NTD) reveal a dramatic reorganization of the highly antigenic N1 loop into a β-strand, providing an explanation for decreased binding of the BA.2 S protein to antibodies isolated from BA.1-convalescent patients. Our analysis reveals structural mechanisms underlying the antigenic drift in the rapidly evolving Omicron variant landscape.
リンク	Cell Rep / PubMed:36640338 / PubMed Central
手法	EM (単粒子)
解像度	2.37 - 3.04 Å
構造データ	27523 8dm1 EMDB-27523, PDB-8dm1: Cryo-EM structure of SARS-CoV-2 Omicron BA.2 spike protein 手法: EM (単粒子) / 解像度: 3.04 Å 27524 8dm2 EMDB-27524, PDB-8dm2: Cryo-EM structure of SARS-CoV-2 Omicron BA.2 spike protein (focused refinement of NTD) 手法: EM (単粒子) / 解像度: 2.91 Å 27525 8dm3 EMDB-27525, PDB-8dm3: Cryo-EM structure of SARS-CoV-2 Omicron BA.2 spike protein in complex with Fab 4A8 手法: EM (単粒子) / 解像度: 2.37 Å 27526 8dm4 EMDB-27526, PDB-8dm4: Cryo-EM structure of SARS-CoV-2 Omicron BA.2 spike protein in complex with Fab 4A8 (focused refinement of NTD and 4A8) 手法: EM (単粒子) / 解像度: 2.45 Å 27527 8dm5 EMDB-27527, PDB-8dm5: Cryo-EM structure of SARS-CoV-2 Omicron BA.2 spike protein in complex with human ACE2 手法: EM (単粒子) / 解像度: 2.51 Å 27528 8dm6 EMDB-27528, PDB-8dm6: Cryo-EM structure of SARS-CoV-2 Omicron BA.2 spike protein in complex with human ACE2 (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 2.77 Å 27529 8dm7 EMDB-27529, PDB-8dm7: Cryo-EM structure of SARS-CoV-2 Omicron BA.2 spike protein in complex with mouse ACE2 手法: EM (単粒子) / 解像度: 2.49 Å 27530 8dm8 EMDB-27530, PDB-8dm8: Cryo-EM structure of SARS-CoV-2 Omicron BA.2 spike protein in complex with mouse ACE2 (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 2.68 Å 27531 8dm9 EMDB-27531, PDB-8dm9: Cryo-EM structure of SARS-CoV-2 Omicron BA.1 spike protein in complex with mouse ACE2 手法: EM (単粒子) / 解像度: 2.56 Å 27532 8dma EMDB-27532, PDB-8dma: Cryo-EM structure of SARS-CoV-2 Omicron BA.1 spike protein in complex with mouse ACE2 (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 2.79 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose
由来	severe acute respiratory syndrome coronavirus 2 (ウイルス) homo sapiens (ヒト) mus musculus (ハツカネズミ)
キーワード	VIRAL PROTEIN / SARS-CoV-2 / glycoprotein / fusion protein / Omicron / BA.2 / NTD / VIRAL PROTEIN/IMMUNE SYSTEM / Viral Protein-Immune System complex / 4A8 / ACE2 / mouse / BA.1