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TitleCorrection: Fluorescence activation mechanism and imaging of drug permeation with new sensors for smoking-cessation ligands.
Journal, issue, pagesElife, Vol. 11, Year 2022
Publish dateSep 17, 2021 (structure data deposition date)
AuthorsNichols, A.L. / Blumenfeld, Z. / Fan, C. / Luebbert, L. / Blom, A.E.M. / Cohen, B.N. / Marvin, J.S. / Borden, P.M. / Kim, C.H. / Muthusamy, A.K. ...Nichols, A.L. / Blumenfeld, Z. / Fan, C. / Luebbert, L. / Blom, A.E.M. / Cohen, B.N. / Marvin, J.S. / Borden, P.M. / Kim, C.H. / Muthusamy, A.K. / Shivange, A.V. / Knox, H.J. / Campello, H.R. / Wang, J.H. / Dougherty, D.A. / Looger, L.L. / Gallagher, T. / Rees, D.C. / Lester, H.A.
External linksElife / PubMed:36520038
MethodsX-ray diffraction
Resolution2.5 - 3.2 Å
Structure data

PDB-7s7t:
iNicSnFR3a Nicotine Sensor comprising Periplasmic Binding sequence plus Fluorescent Sequence with varenicline bound
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

PDB-7s7u:
Crystal structure of iNicSnFR3a Fluorescent Nicotine Sensor with nicotine bound
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

PDB-7s7v:
Crystal structure of iNicSnFR3a Fluorescent Nicotine Sensor
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-QMR:
VARENICLINE / medication, agonist*YM

ChemComp-IOD:
IODIDE ION

ChemComp-HOH:
WATER

Source
  • thermoanaerobacter sp. x513 (bacteria)
KeywordsFLUORESCENT PROTEIN / nicotine / gfp / periplasmic binding protein / nicotine-binding protein / fluorescent sensor / pharmacokinetic sensor / CHOLINE-BINDING PROTEIN

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