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TitleInsights into the importance of WPD-loop sequence for activity and structure in protein tyrosine phosphatases.
Journal, issue, pagesChem Sci, Vol. 13, Page 13524-13540, Year 2022
Publish dateJun 12, 2020 (structure data deposition date)
AuthorsShen, R. / Crean, R.M. / Olsen, K.J. / Corbella, M. / Calixto, A.R. / Richan, T. / Brandao, T.A.S. / Berry, R.D. / Tolman, A. / Loria, J.P. ...Shen, R. / Crean, R.M. / Olsen, K.J. / Corbella, M. / Calixto, A.R. / Richan, T. / Brandao, T.A.S. / Berry, R.D. / Tolman, A. / Loria, J.P. / Johnson, S.J. / Kamerlin, S.C.L. / Hengge, A.C.
External linksChem Sci / PubMed:36507179
MethodsX-ray diffraction
Resolution1.549 - 2.549 Å
Structure data

PDB-6xe8:
Crystal Structure of the PTP1B YopH WPD loop Chimera 3 apo form
Method: X-RAY DIFFRACTION / Resolution: 1.952 Å

PDB-6xea:
Crystal Structure of the PTP1B YopH WPD loop Chimera 3 bound to vanadate
Method: X-RAY DIFFRACTION / Resolution: 1.549 Å

PDB-6xed:
Crystal Structure of the PTP1B YopH WPD loop Chimera 3 bound to tungstate
Method: X-RAY DIFFRACTION / Resolution: 1.795 Å

PDB-6xee:
Crystal Structure of the PTP1B YopH WPD loop Chimera 4 apo form
Method: X-RAY DIFFRACTION / Resolution: 2.501 Å

PDB-6xef:
Crystal structure of the PTP1B YopH WPD loop Chimera 4 bound to vanadate
Method: X-RAY DIFFRACTION / Resolution: 2.048 Å

PDB-6xeg:
Crystal structure of the PTP1B YopH WPD loop Chimera 4 bound to tungstate
Method: X-RAY DIFFRACTION / Resolution: 2.549 Å

PDB-7s4f:
Protein Tyrosine Phosphatase 1B - F182Q mutant bound with Hepes
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

Chemicals

ChemComp-BEN:
BENZAMIDINE

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-VO4:
VANADATE ION

ChemComp-WO4:
TUNGSTATE(VI)ION

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-GOL:
GLYCEROL

Source
  • homo sapiens (human)
KeywordsHYDROLASE / PTP1B / tyrosine phosphatase / WPD loop / STRUCTURAL PROTEIN / P-loop / WPD-loops / Hepes / Protein Phosphatase / EGFR receptor / Phosphoprotein

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