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TitleVibrio parahaemolyticus prey targeting requires autoproteolysis-triggered dimerization of the type VI secretion system effector RhsP.
Journal, issue, pagesCell Rep, Vol. 41, Issue 10, Page 111732, Year 2022
Publish dateDec 6, 2022
AuthorsLe Tang / Shuqi Dong / Nadia Rasheed / Hao Weng Wu / Ningkun Zhou / Huadong Li / Meilin Wang / Jun Zheng / Jun He / William Chong Hang Chao /
PubMed AbstractThe rearrangement hotspot (Rhs) repeat is an ancient giant protein fold found in all domains of life. Rhs proteins are polymorphic toxins that could either be deployed as an ABC complex or via a type ...The rearrangement hotspot (Rhs) repeat is an ancient giant protein fold found in all domains of life. Rhs proteins are polymorphic toxins that could either be deployed as an ABC complex or via a type VI secretion system (T6SS) in interbacterial competitions. To explore the mechanism of T6SS-delivered Rhs toxins, we used the gastroenteritis-associated Vibrio parahaemolyticus as a model organism and identified an Rhs toxin-immunity pair, RhsP-RhsP. Our data show that RhsP-dependent prey targeting by V. parahaemolyticus requires T6SS2. RhsP can bind to VgrG2 independently without a chaperone and spontaneously self-cleaves into three fragments. The toxic C-terminal fragment (RhsP) can bind to VgrG2 via a VgrG2-interacting region (VIR). Our electron microscopy (EM) analysis reveals that the VIR is encapsulated inside the Rhs β barrel structure and that autoproteolysis triggers a dramatic conformational change of the VIR. This alternative VIR conformation promotes RhsP dimerization, which significantly contributes to T6SS2-mediated prey targeting by V. parahaemolyticus.
External linksCell Rep / PubMed:36476863
MethodsEM (single particle)
Resolution3.16 - 3.36 Å
Structure data

34540

8h8a

EMDB-34540, PDB-8h8a:
Type VI secretion system effector RhsP in its post-autoproteolysis and monomeric form
Method: EM (single particle) / Resolution: 3.25 Å

34541

8h8b

EMDB-34541, PDB-8h8b:
Type VI secretion system effector RhsP in its pre-autoproteolysis and monomeric form
Method: EM (single particle) / Resolution: 3.16 Å

34542

8h8c

EMDB-34542, PDB-8h8c:
Type VI secretion system effector RhsP in its post-autoproteolysis and dimeric form
Method: EM (single particle) / Resolution: 3.36 Å

Source
  • vibrio parahaemolyticus serotype o3:k6 (strain rimd 2210633) (bacteria)
  • Vibrio parahaemolyticus (bacteria)
KeywordsTOXIN / T6SS / Rhs proteins / polymorphic toxins

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