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TitleSV40 T-antigen uses a DNA shearing mechanism to initiate origin unwinding.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 119, Issue 49, Page e2216240119, Year 2022
Publish dateDec 6, 2022
AuthorsLance D Langston / Zuanning Yuan / Roxana Georgescu / Huilin Li / Michael E O'Donnell /
PubMed AbstractDuplication of DNA genomes requires unwinding of the double-strand (ds) DNA so that each single strand (ss) can be copied by a DNA polymerase. The genomes of eukaryotic cells are unwound by two ring- ...Duplication of DNA genomes requires unwinding of the double-strand (ds) DNA so that each single strand (ss) can be copied by a DNA polymerase. The genomes of eukaryotic cells are unwound by two ring-shaped hexameric helicases that initially encircle dsDNA but transition to ssDNA for function as replicative helicases. How the duplex is initially unwound, and the role of the two helicases in this process, is poorly understood. We recently described an initiation mechanism for eukaryotes in which the two helicases are directed inward toward one another and shear the duplex open by pulling on opposite strands of the duplex while encircling dsDNA [L. D. Langston, M. E. O'Donnell, , e46515 (2019)]. Two head-to-head T-Antigen helicases are long known to be loaded at the SV40 origin. We show here that T-Antigen tracks head (N-tier) first on ssDNA, opposite the direction proposed for decades. We also find that SV40 T-Antigen tracks directionally while encircling dsDNA and mainly tracks on one strand of the duplex in the same orientation as during ssDNA translocation. Further, two inward directed T-Antigen helicases on dsDNA are able to melt a 150-bp duplex. These findings explain the "rabbit ear" DNA loops observed at the SV40 origin by electron microscopy and reconfigure how the DNA loops emerge from the double hexamer relative to earlier models. Thus, the mechanism of DNA shearing by two opposing helicases is conserved in a eukaryotic viral helicase and may be widely used to initiate origin unwinding of dsDNA genomes.
External linksProc Natl Acad Sci U S A / PubMed:36442086 / PubMed Central
MethodsEM (single particle)
Resolution5.8 Å
Structure data

EMDB-28195: SV40 T-Antigen helicase hexamer
Method: EM (single particle) / Resolution: 5.8 Å

Source
  • Betapolyomavirus macacae

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