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-Structure paper
Title | An epitope-enriched immunogen expands responses to a conserved viral site. |
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Journal, issue, pages | Cell Rep, Vol. 41, Issue 6, Page 111628, Year 2022 |
Publish date | Nov 8, 2022 |
Authors | Timothy M Caradonna / Larance Ronsard / Ashraf S Yousif / Ian W Windsor / Rachel Hecht / Thalia Bracamonte-Moreno / Anne A Roffler / Max J Maron / Daniel P Maurer / Jared Feldman / Elisa Marchiori / Ralston M Barnes / Daniel Rohrer / Nils Lonberg / Thomas H Oguin / Gregory D Sempowski / Thomas B Kepler / Masayuki Kuraoka / Daniel Lingwood / Aaron G Schmidt / |
PubMed Abstract | Pathogens evade host humoral responses by accumulating mutations in surface antigens. While variable, there are conserved regions that cannot mutate without compromising fitness. Antibodies targeting ...Pathogens evade host humoral responses by accumulating mutations in surface antigens. While variable, there are conserved regions that cannot mutate without compromising fitness. Antibodies targeting these conserved epitopes are often broadly protective but remain minor components of the repertoire. Rational immunogen design leverages a structural understanding of viral antigens to modulate humoral responses to favor these responses. Here, we report an epitope-enriched immunogen presenting a higher copy number of the influenza hemagglutinin (HA) receptor-binding site (RBS) epitope relative to other B cell epitopes. Immunization in a partially humanized murine model imprinted with an H1 influenza shows H1-specific serum and >99% H1-specific B cells being RBS-directed. Single B cell analyses show a genetically restricted response that structural analysis defines as RBS-directed antibodies engaging the RBS with germline-encoded contacts. These data show how epitope enrichment expands B cell responses toward conserved epitopes and advances immunogen design approaches for next-generation viral vaccines. |
External links | Cell Rep / PubMed:36351401 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.36 - 5.0 Å |
Structure data | EMDB-26604: H1 Solomon Islands 2006 hemagglutinin in complex with Ab111 EMDB-26605, PDB-7umm: |
Chemicals | ChemComp-NAG: |
Source |
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Keywords | VIRAL PROTEIN / influenza / antibody / vaccine |