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TitleProduction and Characterisation of Stabilised PV-3 Virus-like Particles Using .
Journal, issue, pagesViruses, Vol. 14, Issue 10, Year 2022
Publish dateSep 30, 2022
AuthorsLee Sherry / Keith Grehan / Jessica J Swanson / Mohammad W Bahar / Claudine Porta / Elizabeth E Fry / David I Stuart / David J Rowlands / Nicola J Stonehouse /
PubMed AbstractFollowing the success of global vaccination programmes using the live-attenuated oral and inactivated poliovirus vaccines (OPV and IPV), wild poliovirus (PV) is now only endemic in Afghanistan and ...Following the success of global vaccination programmes using the live-attenuated oral and inactivated poliovirus vaccines (OPV and IPV), wild poliovirus (PV) is now only endemic in Afghanistan and Pakistan. However, the continued use of these vaccines poses potential risks to the eradication of PV. The production of recombinant PV virus-like particles (VLPs), which lack the viral genome offer great potential as next-generation vaccines for the post-polio world. We have previously reported production of PV VLPs using , however, these VLPs were in the non-native conformation (C Ag), which would not produce effective protection against PV. Here, we build on this work and show that it is possible to produce wt PV-3 and thermally stabilised PV-3 (referred to as PV-3 SC8) VLPs in the native conformation (D Ag) using . We show that the PV-3 SC8 VLPs provide a much-improved D:C antigen ratio as compared to wt PV-3, whilst exhibiting greater thermostability than the current IPV vaccine. Finally, we determine the cryo-EM structure of the yeast-derived PV-3 SC8 VLPs and compare this to previously published PV-3 D Ag structures, highlighting the similarities between these recombinantly expressed VLPs and the infectious virus, further emphasising their potential as a next-generation vaccine candidate for PV.
External linksViruses / PubMed:36298714 / PubMed Central
MethodsEM (single particle)
Resolution2.7 Å
Structure data

15543

8anw

EMDB-15543, PDB-8anw:
Poliovirus type 3 (strain Saukett) stabilised virus-like particle (PV3 SC8).
Method: EM (single particle) / Resolution: 2.7 Å

Chemicals

ChemComp-SPH:
SPHINGOSINE

Source
  • human poliovirus 3
KeywordsVIRUS LIKE PARTICLE / Capsid protein

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