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-Structure paper
タイトル | Coinfection by influenza A virus and respiratory syncytial virus produces hybrid virus particles. |
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ジャーナル・号・ページ | Nat Microbiol, Vol. 7, Issue 11, Page 1879-1890, Year 2022 |
掲載日 | 2022年10月24日 |
著者 | Joanne Haney / Swetha Vijayakrishnan / James Streetley / Kieran Dee / Daniel Max Goldfarb / Mairi Clarke / Margaret Mullin / Stephen D Carter / David Bhella / Pablo R Murcia / |
PubMed 要旨 | Interactions between respiratory viruses during infection affect transmission dynamics and clinical outcomes. To identify and characterize virus-virus interactions at the cellular level, we ...Interactions between respiratory viruses during infection affect transmission dynamics and clinical outcomes. To identify and characterize virus-virus interactions at the cellular level, we coinfected human lung cells with influenza A virus (IAV) and respiratory syncytial virus (RSV). Super-resolution microscopy, live-cell imaging, scanning electron microscopy and cryo-electron tomography revealed extracellular and membrane-associated filamentous structures consistent with hybrid viral particles (HVPs). We found that HVPs harbour surface glycoproteins and ribonucleoproteins of IAV and RSV. HVPs use the RSV fusion glycoprotein to evade anti-IAV neutralizing antibodies and infect and spread among cells lacking IAV receptors. Finally, we show that IAV and RSV coinfection in primary cells of the bronchial epithelium results in viral proteins from both viruses co-localizing at the apical cell surface. Our observations define a previously unknown interaction between respiratory viruses that might affect virus pathogenesis by expanding virus tropism and enabling immune evasion. |
リンク | Nat Microbiol / PubMed:36280786 |
手法 | EM (トモグラフィー) |
構造データ | EMDB-13228: Cryo-electron tomogram of a hybrid virus particle generated by coinfection of human Influenza A virus and Respiratory Syncytial virus on lung cells. EMDB-13229: Cryo-electron tomogram of a pseudotype virus particle generated during coinfection of Influenza A virus and Respiratory Syncytial virus in lung cells. |