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-Structure paper
Title | Identification and receptor mechanism of TIR-catalyzed small molecules in plant immunity. |
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Journal, issue, pages | Science, Vol. 377, Issue 6605, Page eabq3297, Year 2022 |
Publish date | Jul 29, 2022 |
Authors | Shijia Huang / Aolin Jia / Wen Song / Giuliana Hessler / Yonggang Meng / Yue Sun / Lina Xu / Henriette Laessle / Jan Jirschitzka / Shoucai Ma / Yu Xiao / Dongli Yu / Jiao Hou / Ruiqi Liu / Huanhuan Sun / Xiaohui Liu / Zhifu Han / Junbiao Chang / Jane E Parker / Jijie Chai / |
PubMed Abstract | Plant nucleotide-binding leucine-rich repeat-containing (NLR) receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain sense pathogen effectors to enable TIR-encoded nicotinamide adenine ...Plant nucleotide-binding leucine-rich repeat-containing (NLR) receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain sense pathogen effectors to enable TIR-encoded nicotinamide adenine dinucleotide hydrolase (NADase) activity for immune signaling. TIR-NLR signaling requires the helper NLRs N requirement gene 1 (NRG1), Activated Disease Resistance 1 (ADR1), and Enhanced Disease Susceptibility 1 (EDS1), which forms a heterodimer with each of its paralogs Phytoalexin Deficient 4 (PAD4) and Senescence-Associated Gene 101 (SAG101). Here, we show that TIR-containing proteins catalyze the production of 2'-(5''-phosphoribosyl)-5'-adenosine monophosphate (pRib-AMP) and diphosphate (pRib-ADP) in vitro and in planta. Biochemical and structural data demonstrate that EDS1-PAD4 is a receptor complex for pRib-AMP and pRib-ADP, which allosterically promote EDS1-PAD4 interaction with ADR1-L1 but not NRG1A. Our study identifies TIR-catalyzed pRib-AMP and pRib-ADP as a missing link in TIR signaling through EDS1-PAD4 and as likely second messengers for plant immunity. |
External links | Science / PubMed:35857645 |
Methods | EM (single particle) / X-ray diffraction |
Resolution | 2.29 - 2.93 Å |
Structure data | EMDB-33144, PDB-7xdd: PDB-7xey: |
Chemicals | ChemComp-ADP: ChemComp-RP5: ChemComp-TRS: ChemComp-HOH: |
Source |
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Keywords | TRANSFERASE/TRANSFERASE ACTIVATOR / NLR / Plant Protein / Plant immune signaling / TRANSFERASE-TRANSFERASE ACTIVATOR complex / EDS1 / PAD4 / pRib-ADP |