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TitleCryo-EM structure of a monomeric yeast S. cerevisiae complex IV isolated with maltosides: Implications in supercomplex formation.
Journal, issue, pagesBiochim Biophys Acta Bioenerg, Vol. 1863, Issue 7, Page 148591, Year 2022
Publish dateJul 14, 2022
AuthorsGabriel Ing / Andrew M Hartley / Nikos Pinotsis / Amandine Maréchal /
PubMed AbstractIn mitochondria, complex IV (CIV) can be found as a monomer, a dimer or in association with other respiratory complexes. The atomic structure of the yeast S. cerevisiae CIV in a supercomplex (SC) ...In mitochondria, complex IV (CIV) can be found as a monomer, a dimer or in association with other respiratory complexes. The atomic structure of the yeast S. cerevisiae CIV in a supercomplex (SC) with complex III (CIII) pointed to a region of significant conformational changes compared to the homologous mammalian CIV structures. These changes involved the matrix side domain of Cox5A at the CIII-CIV interface, and it was suggested that it could be required for SC formation. To investigate this, we solved the structure of the isolated monomeric CIV from S. cerevisiae stabilised in amphipol A8-35 at 3.9 Å using cryo-electron microscopy. Only a minor change in flexibility was seen in this Cox5A region, ruling out large CIV conformational shift for interaction with CIII and confirming the different fold of the yeast Cox5A subunit compared to mammalian homologues. Other differences in structure were the absence of two canonical subunits, Cox12 and Cox13, as well as Cox26, which is unique to the yeast CIV. Their absence is most likely due to the protein purification protocol used to isolate CIV from the III-IV SC.
External linksBiochim Biophys Acta Bioenerg / PubMed:35839926
MethodsEM (single particle)
Resolution3.87 Å
Structure data

14436

7z10

EMDB-14436, PDB-7z10:
Monomeric respiratory complex IV isolated from S. cerevisiae
Method: EM (single particle) / Resolution: 3.87 Å

Chemicals

ChemComp-CU:
COPPER (II) ION

ChemComp-HEA:
HEME-A

ChemComp-MG:
Unknown entry

ChemComp-PEF:
DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE / phospholipid*YM

ChemComp-CUA:
DINUCLEAR COPPER ION

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae s288c (yeast)
KeywordsOXIDOREDUCTASE / Cytochrome C Oxidase / Mitochondria Respiratory Chain / Complex IV / Oxidoreductace-electron transport complex

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