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Title | A transformation clustering algorithm and its application in polyribosomes structural profiling. |
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Journal, issue, pages | Nucleic Acids Res, Vol. 50, Issue 16, Page 9001-9011, Year 2022 |
Publish date | Sep 9, 2022 |
Authors | Wenhong Jiang / Jonathan Wagner / Wenjing Du / Juergen Plitzko / Wolfgang Baumeister / Florian Beck / Qiang Guo / |
PubMed Abstract | Improvements in cryo-electron tomography sample preparation, electron-microscopy instrumentations, and image processing algorithms have advanced the structural analysis of macromolecules in situ. ...Improvements in cryo-electron tomography sample preparation, electron-microscopy instrumentations, and image processing algorithms have advanced the structural analysis of macromolecules in situ. Beyond such analyses of individual macromolecules, the study of their interactions with functionally related neighbors in crowded cellular habitats, i.e. 'molecular sociology', is of fundamental importance in biology. Here we present a NEighboring Molecule TOpology Clustering (NEMO-TOC) algorithm. We optimized this algorithm for the detection and profiling of polyribosomes, which play both constitutive and regulatory roles in gene expression. Our results suggest a model where polysomes are formed by connecting multiple nonstochastic blocks, in which translation is likely synchronized. |
External links | Nucleic Acids Res / PubMed:35811088 / PubMed Central |
Methods | EM (subtomogram averaging) / EM (tomography) |
Resolution | 12.3 - 42.5 Å |
Structure data | EMDB-33018: the neuron ribosome bound to the membrane EMDB-33072: the tomogram of neuron cortical cell EMDB-33073: one tomogram of the neuron cortical cell EMDB-33074: neighboring ribosomes cluster1 EMDB-33076: neighboring ribosomes of cluster3 EMDB-33077: ribosome translation conformation 1 EMDB-33081: ribosome translation conformation 5 |
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