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TitleStructure-based electron-confurcation mechanism of the Ldh-EtfAB complex.
Journal, issue, pagesElife, Vol. 11, Year 2022
Publish dateJun 24, 2022
AuthorsKanwal Kayastha / Alexander Katsyv / Christina Himmrich / Sonja Welsch / Jan M Schuller / Ulrich Ermler / Volker Müller /
PubMed AbstractLactate oxidation with NAD as electron acceptor is a highly endergonic reaction. Some anaerobic bacteria overcome the energetic hurdle by flavin-based electron bifurcation/confurcation (FBEB/FBEC) ...Lactate oxidation with NAD as electron acceptor is a highly endergonic reaction. Some anaerobic bacteria overcome the energetic hurdle by flavin-based electron bifurcation/confurcation (FBEB/FBEC) using a lactate dehydrogenase (Ldh) in concert with the electron-transferring proteins EtfA and EtfB. The electron cryo-microscopically characterized (Ldh-EtfAB) complex of at 2.43 Å resolution consists of a mobile EtfAB shuttle domain located between the rigid central Ldh and the peripheral EtfAB base units. The FADs of Ldh and the EtfAB shuttle domain contact each other thereby forming the D (dehydrogenation-connected) state. The intermediary Glu37 and Glu139 may harmonize the redox potentials between the FADs and the pyruvate/lactate pair crucial for FBEC. By integrating Alphafold2 calculations a plausible novel B (bifurcation-connected) state was obtained allowing electron transfer between the EtfAB base and shuttle FADs. Kinetic analysis of enzyme variants suggests a correlation between NAD binding site and D-to-B-state transition implicating a 75° rotation of the EtfAB shuttle domain. The FBEC inactivity when truncating the ferredoxin domain of EtfA substantiates its role as redox relay. Lactate oxidation in Ldh is assisted by the catalytic base His423 and a metal center. On this basis, a comprehensive catalytic mechanism of the FBEC process was proposed.
External linksElife / PubMed:35748623 / PubMed Central
MethodsEM (single particle)
Resolution2.43 Å
Structure data

13960

7qh2

EMDB-13960, PDB-7qh2:
Cryo-EM structure of Ldh-EtfAB complex from Acetobacterium woodii
Method: EM (single particle) / Resolution: 2.43 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM / Flavin adenine dinucleotide

ChemComp-FE:
Unknown entry / Iron

ChemComp-HOH:
WATER / Water

Source
  • acetobacterium woodii (bacteria)
KeywordsFLAVOPROTEIN / Electron bifurcation / Electron confirmation / Lactate / Lactate dehydrogenase complex / Electron transferring flavoprotein / A. woodii / redox enzyme

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