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TitleThe 3D-structure, kinetics and dynamics of the E. coli nitroreductase NfsA with NADP + provide glimpses of its catalytic mechanism.
Journal, issue, pagesFebs Lett., Vol. 596, Page 2425-2440, Year 2022
Publish dateOct 15, 2021 (structure data deposition date)
AuthorsWhite, S.A. / Christofferson, A.J. / Grainger, A.I. / Day, M.A. / Jarrom, D. / Graziano, A.E. / Searle, P.F. / Hyde, E.I.
External linksFebs Lett. / PubMed:35648111
MethodsX-ray diffraction
Resolution0.96 - 2.06 Å
Structure data

PDB-7q0o:
E. coli NfsA
Method: X-RAY DIFFRACTION / Resolution: 0.96 Å

PDB-7z0w:
E. coli NfsA bound to NADP+
Method: X-RAY DIFFRACTION / Resolution: 2.06 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-HOH:
WATER

ChemComp-ATR:
2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE

ChemComp-MG:
Unknown entry

Source
  • escherichia coli k-12 (bacteria)
KeywordsOXIDOREDUCTASE / Oxygen-insensitive NADPH nitroreductase / NADP+ complex

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