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Structure paper

TitleL-tyrosine-bound ThiH structure reveals C-C bond break differences within radical SAM aromatic amino acid lyases.
Journal, issue, pagesNat Commun, Vol. 13, Page 2284-2284, Year 2022
Publish dateAug 4, 2021 (structure data deposition date)
AuthorsAmara, P. / Saragaglia, C. / Mouesca, J.M. / Martin, L. / Nicolet, Y.
External linksNat Commun / PubMed:35477710
MethodsX-ray diffraction
Resolution1.27 - 1.99 Å
Structure data

PDB-7pd1:
Crystal structure of the L-tyrosine-bound radical SAM tyrosine lyase ThiH (2-iminoacetate synthase) from Thermosinus carboxydivorans
Method: X-RAY DIFFRACTION / Resolution: 1.27 Å

PDB-7pd2:
Crystal structure of the substrate-free radical SAM tyrosine lyase ThiH (2-iminoacetate synthase) from Thermosinus carboxydivorans
Method: X-RAY DIFFRACTION / Resolution: 1.99 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-5AD:
5'-DEOXYADENOSINE

ChemComp-MET:
METHIONINE

ChemComp-TYR:
TYROSINE

ChemComp-SO4:
SULFATE ION

ChemComp-K:
Unknown entry

ChemComp-BR:
BROMIDE ION

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • thermosinus carboxydivorans nor1 (bacteria)
KeywordsLYASE / Radical SAM enzyme 2-iminoacetate Metalloprotein

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