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TitleLiquid-to-solid phase transition of oskar ribonucleoprotein granules is essential for their function in Drosophila embryonic development.
Journal, issue, pagesCell, Vol. 185, Issue 8, Page 1308-1324.e23, Year 2022
Publish dateApr 14, 2022
AuthorsMainak Bose / Marko Lampe / Julia Mahamid / Anne Ephrussi /
PubMed AbstractAsymmetric localization of oskar ribonucleoprotein (RNP) granules to the oocyte posterior is crucial for abdominal patterning and germline formation in the Drosophila embryo. We show that oskar RNP ...Asymmetric localization of oskar ribonucleoprotein (RNP) granules to the oocyte posterior is crucial for abdominal patterning and germline formation in the Drosophila embryo. We show that oskar RNP granules in the oocyte are condensates with solid-like physical properties. Using purified oskar RNA and scaffold proteins Bruno and Hrp48, we confirm in vitro that oskar granules undergo a liquid-to-solid phase transition. Whereas the liquid phase allows RNA incorporation, the solid phase precludes incorporation of additional RNA while allowing RNA-dependent partitioning of client proteins. Genetic modification of scaffold granule proteins or tethering the intrinsically disordered region of human fused in sarcoma (FUS) to oskar mRNA allowed modulation of granule material properties in vivo. The resulting liquid-like properties impaired oskar localization and translation with severe consequences on embryonic development. Our study reflects how physiological phase transitions shape RNA-protein condensates to regulate the localization and expression of a maternal RNA that instructs germline formation.
External linksCell / PubMed:35325593 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-14212:
Cryo-electron tomography of in vitro assembled Bruno and oskar 3'UTR RNA condensates
Method: EM (tomography)

EMDB-14213:
Cryo-electron tomography of in vitro assembled Bruno and oskar full length RNA condensates
Method: EM (tomography)

EMDB-14214:
Cryo-electron tomography of in vitro assembled Hrp48 and oskar 3'UTR RNA condensates
Method: EM (tomography)

EMDB-14215:
Cryo-electron tomography of in vitro assembled Bruno and oskar 3'UTR RNA condensates followed by addition of more oskar 3'UTR RNA
Method: EM (tomography)

EMDB-14216:
Cryo-electron tomography of in vitro assembled Bruno oskar 3'UTR RNA condensates aged for 30 minutes followed by addition of more oskar 3'UTR RNA
Method: EM (tomography)

EMDB-14217:
Cryo-electron tomography of in vitro assembled Bruno-EGFP and oskar 3'UTR RNA condensates aged for 30 minutes followed by addition of RFP-PTB
Method: EM (tomography)

Source
  • Drosophila melanogaster (fruit fly)

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