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-Structure paper
Title | Transcription factors modulate RNA polymerase conformational equilibrium. |
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Journal, issue, pages | Nat Commun, Vol. 13, Issue 1, Page 1546, Year 2022 |
Publish date | Mar 22, 2022 |
Authors | Chengjin Zhu / Xieyang Guo / Philippe Dumas / Maria Takacs / Mo'men Abdelkareem / Arnaud Vanden Broeck / Charlotte Saint-André / Gabor Papai / Corinne Crucifix / Julio Ortiz / Albert Weixlbaumer / |
PubMed Abstract | RNA polymerase (RNAP) frequently pauses during the transcription of DNA to RNA to regulate gene expression. Transcription factors NusA and NusG modulate pausing, have opposing roles, but can bind ...RNA polymerase (RNAP) frequently pauses during the transcription of DNA to RNA to regulate gene expression. Transcription factors NusA and NusG modulate pausing, have opposing roles, but can bind RNAP simultaneously. Here we report cryo-EM reconstructions of Escherichia coli RNAP bound to NusG, or NusA, or both. RNAP conformational changes, referred to as swivelling, correlate with transcriptional pausing. NusA facilitates RNAP swivelling to further increase pausing, while NusG counteracts this role. Their structural effects are consistent with biochemical results on two categories of transcriptional pauses. In addition, the structures suggest a cooperative mechanism of NusA and NusG during Rho-mediated transcription termination. Our results provide a structural rationale for the stochastic nature of pausing and termination and how NusA and NusG can modulate it. |
External links | Nat Commun / PubMed:35318334 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.8 - 4.5 Å |
Structure data | EMDB-13706, PDB-7py0: EMDB-13707, PDB-7py1: EMDB-13709, PDB-7py3: EMDB-13713, PDB-7py5: EMDB-13714, PDB-7py6: EMDB-13715, PDB-7py7: EMDB-13716, PDB-7py8: EMDB-13717, PDB-7pyj: EMDB-13718, PDB-7pyk: EMDB-13745, PDB-7q0j: EMDB-13746, PDB-7q0k: |
Chemicals | ChemComp-MG: ChemComp-ZN: |
Source |
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Keywords | TRANSCRIPTION / NusG / transcription elongation / cryo-EM / NusA / NusA and NusG / RNAP transcription elongation |