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-Structure paper
タイトル | Nuclear translocation of vitellogenin in the honey bee (). |
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ジャーナル・号・ページ | Apidologie, Vol. 53, Issue 1, Page 13, Year 2022 |
掲載日 | 2022年3月15日 |
![]() | Heli Salmela / Gyan P Harwood / Daniel Münch / Christine G Elsik / Elías Herrero-Galán / Maria K Vartiainen / Gro V Amdam / ![]() ![]() ![]() ![]() |
PubMed 要旨 | Vitellogenin (Vg) is a conserved protein used by nearly all oviparous animals to produce eggs. It is also pleiotropic and performs functions in oxidative stress resistance, immunity, and, in honey ...Vitellogenin (Vg) is a conserved protein used by nearly all oviparous animals to produce eggs. It is also pleiotropic and performs functions in oxidative stress resistance, immunity, and, in honey bees, behavioral development of the worker caste. It has remained enigmatic how Vg affects multiple traits. Here, we asked whether Vg enters the nucleus and acts via DNA-binding. We used cell fractionation, immunohistology, and cell culture to show that a structural subunit of honey bee Vg translocates into cell nuclei. We then demonstrated Vg-DNA binding theoretically and empirically with prediction software and chromatin immunoprecipitation with sequencing (ChIP-seq), finding binding sites at genes influencing immunity and behavior. Finally, we investigated the immunological and enzymatic conditions affecting Vg cleavage and nuclear translocation and constructed a 3D structural model. Our data are the first to show Vg in the nucleus and suggest a new fundamental regulatory role for this ubiquitous protein. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13592-022-00914-9. |
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手法 | EM (単粒子) |
解像度 | 27.0 Å |
構造データ | ![]() EMDB-22113: |
由来 |
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