EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Near-atomic structure of the inner ring of the Saccharomyces cerevisiae nuclear pore complex.
ジャーナル・号・ページ	Cell Res, Vol. 32, Issue 5, Page 437-450, Year 2022
掲載日	2022年3月18日
著者	Zongqiang Li / Shuaijiabin Chen / Liang Zhao / Guoqiang Huang / Xiong Pi / Shan Sun / Peiyi Wang / Sen-Fang Sui /
PubMed 要旨	Nuclear pore complexes (NPCs) mediate bidirectional nucleocytoplasmic transport of substances in eukaryotic cells. However, the accurate molecular arrangement of NPCs remains enigmatic owing to their ...Nuclear pore complexes (NPCs) mediate bidirectional nucleocytoplasmic transport of substances in eukaryotic cells. However, the accurate molecular arrangement of NPCs remains enigmatic owing to their huge size and highly dynamic nature. Here we determined the structure of the asymmetric unit of the inner ring (IR monomer) at 3.73 Å resolution by single-particle cryo-electron microscopy, and created an atomic model of the intact IR consisting of 192 molecules of 8 nucleoporins. In each IR monomer, the Z-shaped Nup188-Nup192 complex in the middle layer is sandwiched by two approximately parallel rhomboidal structures in the inner and outer layers, while Nup188, Nup192 and Nic96 link all subunits to constitute a relatively stable IR monomer. In contrast, the intact IR is assembled by loose and instable interactions between IR monomers. These structures, together with previously reported structural information of IR, reveal two distinct interaction modes between IR monomers and extensive flexible connections in IR assembly, providing a structural basis for the stability and malleability of IR.
リンク	Cell Res / PubMed:35301440 / PubMed Central
手法	EM (単粒子)
解像度	2.81 - 12.03 Å
構造データ	32643 7wo9 EMDB-32643, PDB-7wo9: Cryo-EM structure of full-length Nup188 手法: EM (単粒子) / 解像度: 2.81 Å EMDB-32644: Cryo-EM structure of full-length Nup188 (multiBody refined N-terminal region) 手法: EM (単粒子) / 解像度: 3.02 Å EMDB-32645: Cryo-EM structure of full-length Nup188 (multibody refined C-terminal region) 手法: EM (単粒子) / 解像度: 3.41 Å 32653 7woo EMDB-32653, PDB-7woo: Cryo-EM structure of the inner ring protomer of the Saccharomyces cerevisiae nuclear pore complex 手法: EM (単粒子) / 解像度: 3.71 Å 32658 7wot EMDB-32658, PDB-7wot: Cryo-EM structure of the inner ring monomer of the Saccharomyces cerevisiae nuclear pore complex 手法: EM (単粒子) / 解像度: 3.73 Å EMDB-32662: Cryo-EM map of the inner ring dimer of the Saccharomyces cerevisiae nuclear pore complex 手法: EM (単粒子) / 解像度: 7.69 Å EMDB-32663: Cryo-EM map of the intact inner ring of the Saccharomyces cerevisiae nuclear pore complex 手法: EM (単粒子) / 解像度: 9.1 Å EMDB-32664: Cryo-EM map of the whole Saccharomyces cerevisiae nuclear pore complex 手法: EM (単粒子) / 解像度: 12.03 Å
由来	saccharomyces cerevisiae s288c (パン酵母) saccharomyces cerevisiae (パン酵母) baker's yeast (パン酵母)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / Nup188 / cryo-EM (低温電子顕微鏡法) / Saccharomyces cerevisiae (出芽酵母) / nuclear pore complex (核膜孔) / inner ring (C・S・ルイス) / protomer / monomer (モノマー)