EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures reveal multiple stages of bacterial outer membrane protein folding.
ジャーナル・号・ページ	Cell, Vol. 185, Issue 7, Page 1143-1156.e13, Year 2022
掲載日	2022年3月31日
著者	Matthew Thomas Doyle / John R Jimah / Tyrone Dowdy / Shannon I Ohlemacher / Mioara Larion / Jenny E Hinshaw / Harris D Bernstein /
PubMed 要旨	Transmembrane β barrel proteins are folded into the outer membrane (OM) of Gram-negative bacteria by the β barrel assembly machinery (BAM) via a poorly understood process that occurs without known ...Transmembrane β barrel proteins are folded into the outer membrane (OM) of Gram-negative bacteria by the β barrel assembly machinery (BAM) via a poorly understood process that occurs without known external energy sources. Here, we used single-particle cryo-EM to visualize the folding dynamics of a model β barrel protein (EspP) by BAM. We found that BAM binds the highly conserved "β signal" motif of EspP to correctly orient β strands in the OM during folding. We also found that the folding of EspP proceeds via "hybrid-barrel" intermediates in which membrane integrated β sheets are attached to the essential BAM subunit, BamA. The structures show an unprecedented deflection of the membrane surrounding the EspP intermediates and suggest that β sheets progressively fold toward BamA to form a β barrel. Along with in vivo experiments that tracked β barrel folding while the OM tension was modified, our results support a model in which BAM harnesses OM elasticity to accelerate β barrel folding.
リンク	Cell / PubMed:35294859 / PubMed Central
手法	EM (単粒子)
解像度	3.6 - 4.8 Å
構造データ	26105 7tsz EMDB-26105, PDB-7tsz: BamABCDE bound to substrate EspP class 1 手法: EM (単粒子) / 解像度: 4.5 Å 26106 7tt0 EMDB-26106, PDB-7tt0: BamABCDE bound to substrate EspP class 2 手法: EM (単粒子) / 解像度: 4.3 Å 26107 7tt1 EMDB-26107, PDB-7tt1: BamABCDE bound to substrate EspP class 4 手法: EM (単粒子) / 解像度: 4.3 Å 26108 7tt2 EMDB-26108, PDB-7tt2: BamABCDE bound to substrate EspP class 3 手法: EM (単粒子) / 解像度: 4.2 Å 26109 7tt3 EMDB-26109, PDB-7tt3: BamABCDE bound to substrate EspP class 5 手法: EM (単粒子) / 解像度: 4.3 Å 26110 7tt4 EMDB-26110, PDB-7tt4: BamABCDE bound to substrate EspP class 6 手法: EM (単粒子) / 解像度: 4.2 Å 26111 7tt5 EMDB-26111, PDB-7tt5: BamABCDE bound to substrate EspP in the open-sheet EspP state 手法: EM (単粒子) / 解像度: 4.3 Å 26112 7tt6 EMDB-26112, PDB-7tt6: BamABCDE bound to substrate EspP in the intermediate-open EspP state 手法: EM (単粒子) / 解像度: 4.3 Å 26113 7tt7 EMDB-26113, PDB-7tt7: BamABCDE bound to substrate EspP in the barrelized EspP/continuous open BamA state 手法: EM (単粒子) / 解像度: 4.8 Å 26114 7ttc EMDB-26114, PDB-7ttc: BamABCDE bound to substrate EspP 手法: EM (単粒子) / 解像度: 3.6 Å
化合物	ChemComp-K33: 2,3-dideoxy-6-O-[2-deoxy-4-O-phosphono-2-(tetradecanoylamino)-alpha-L-gulopyranosyl]-1-O-phosphono-beta-D-threo-hexopyranose
由来	escherichia coli (大腸菌)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / membrane protein folding (生体膜) / membrane dynamics / outer membrane protein / BAM / beta-barrel (Βバレル)