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TitleRibosome collisions induce mRNA cleavage and ribosome rescue in bacteria.
Journal, issue, pagesNature, Vol. 603, Issue 7901, Page 503-508, Year 2022
Publish dateMar 9, 2022
AuthorsKazuki Saito / Hanna Kratzat / Annabelle Campbell / Robert Buschauer / A Maxwell Burroughs / Otto Berninghausen / L Aravind / Rachel Green / Roland Beckmann / Allen R Buskirk /
PubMed AbstractRibosome rescue pathways recycle stalled ribosomes and target problematic mRNAs and aborted proteins for degradation. In bacteria, it remains unclear how rescue pathways distinguish ribosomes stalled ...Ribosome rescue pathways recycle stalled ribosomes and target problematic mRNAs and aborted proteins for degradation. In bacteria, it remains unclear how rescue pathways distinguish ribosomes stalled in the middle of a transcript from actively translating ribosomes. Here, using a genetic screen in Escherichia coli, we discovered a new rescue factor that has endonuclease activity. SmrB cleaves mRNAs upstream of stalled ribosomes, allowing the ribosome rescue factor tmRNA (which acts on truncated mRNAs) to rescue upstream ribosomes. SmrB is recruited to ribosomes and is activated by collisions. Cryo-electron microscopy structures of collided disomes from E. coli and Bacillus subtilis show distinct and conserved arrangements of individual ribosomes and the composite SmrB-binding site. These findings reveal the underlying mechanisms by which ribosome collisions trigger ribosome rescue in bacteria.
External linksNature / PubMed:35264790 / PubMed Central
MethodsEM (single particle)
Resolution3.37 - 13.29 Å
Structure data

13952

7qg8

EMDB-13952, PDB-7qg8:
Structure of the collided E. coli disome - VemP-stalled 70S ribosome
Method: EM (single particle) / Resolution: 3.97 Å

13955

7qgh

EMDB-13955, PDB-7qgh:
Structure of the E. coli disome - collided 70S ribosome
Method: EM (single particle) / Resolution: 4.48 Å

13956

7qgn

EMDB-13956, PDB-7qgn:
Structure of the SmrB-bound E. coli disome - stalled 70S ribosome
Method: EM (single particle) / Resolution: 3.37 Å

13958

7qgr

EMDB-13958, PDB-7qgr:
Structure of the SmrB-bound E. coli disome - collided 70S ribosome
Method: EM (single particle) / Resolution: 5.7 Å

13959

7qgu

EMDB-13959, PDB-7qgu:
Structure of the B. subtilis disome - stalled 70S ribosome
Method: EM (single particle) / Resolution: 4.75 Å

13961

7qh4

EMDB-13961, PDB-7qh4:
Structure of the B. subtilis disome - collided 70S ribosome
Method: EM (single particle) / Resolution: 5.45 Å

EMDB-13964:
Structure of the E. coli collided trisome
Method: EM (single particle) / Resolution: 13.29 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-HOH:
WATER

Source
  • escherichia coli k-12 (bacteria)
  • vibrio alginolyticus (bacteria)
  • escherichia coli (E. coli)
  • escherichia coli umea 3162-1 (bacteria)
  • bacillus subtilis (bacteria)
KeywordsRIBOSOME / Ribosome rescue / disome / ribosome collision / stalling / no-go complex / nuclease / SmrB

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