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Structure paper

TitleStructure of a B 12 -dependent radical SAM enzyme in carbapenem biosynthesis.
Journal, issue, pagesNature, Vol. 602, Page 343-348, Year 2022
Publish dateOct 9, 2020 (structure data deposition date)
AuthorsKnox, H.L. / Sinner, E.K. / Townsend, C.A. / Boal, A.K. / Booker, S.J.
External linksNature / PubMed:35110734
MethodsX-ray diffraction
Resolution1.791 - 1.939 Å
Structure data

PDB-7kdx:
Crystal structure of Streptomyces tokunonesis TokK with hydroxycobalamin, 5'-deoxyadenosine, and methionine
Method: X-RAY DIFFRACTION / Resolution: 1.791 Å

PDB-7kdy:
Crystal structure of Streptomyces tokunonesis TokK with hydroxycobalamin, 5'-deoxyadenosine, methionine, and (2R)-pantetheinylated carbapenam
Method: X-RAY DIFFRACTION / Resolution: 1.939 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-5AD:
5'-DEOXYADENOSINE

ChemComp-B12:
COBALAMIN

ChemComp-MET:
METHIONINE

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-K:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-WCD:
(2R,3R,5R)-3-{[2-({N-[(2R)-2,4-dihydroxy-3,3-dimethylbutanoyl]-beta-alanyl}amino)ethyl]sulfanyl}-7-oxo-1-azabicyclo[3.2.0]heptane-2-carboxylic acid

ChemComp-GOL:
GLYCEROL

Source
  • streptomyces tokunonensis (bacteria)
KeywordsTRANSFERASE / Radical SAM / Cobalamin / [4Fe-4S] cluster / FeS cluster / methyltransferase / B12 / beta-lactam antibiotic synthesis

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