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Structure paper

TitleStructural Basis for Blocked Excited State Proton Transfer in a Fluorescent, Photoacidic Non-Canonical Amino Acid-Containing Antibody Fragment.
Journal, issue, pagesJ. Mol. Biol., Vol. 434, Page 167455-167455, Year 2022
Publish dateSep 30, 2021 (structure data deposition date)
AuthorsHenderson, J.N. / Simmons, C.R. / Mills, J.H.
External linksJ. Mol. Biol. / PubMed:35033559
MethodsX-ray diffraction
Resolution2 - 2.09 Å
Structure data

PDB-7sen:
Crystal structure of Fab containing a fluorescent noncanonical amino acid with blocked excited state proton transfer
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

PDB-7sgm:
Crystal structure of a Fab variant containing a fluorescent noncanonical amino acid with blocked excited state proton transfer and in complex with its antigen, CD40L
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-TMO:
trimethylamine oxide

Source
  • homo sapiens (human)
KeywordsIMMUNE SYSTEM / Fragment antigen binding (Fab) / noncanonical amino acid / 7-hydroxycoumarin / CYTOKINE/IMMUNE SYSTEM / CYTOKINE-IMMUNE SYSTEM complex

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