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TitleReaction pathway engineering converts a radical hydroxylase into a halogenase.
Journal, issue, pagesNat. Chem. Biol., Vol. 18, Page 171-179, Year 2022
Publish dateAug 14, 2020 (structure data deposition date)
AuthorsNeugebauer, M.E. / Kissman, E.N. / Marchand, J.A. / Pelton, J.G. / Sambold, N.A. / Millar, D.C. / Chang, M.C.Y.
External linksNat. Chem. Biol. / PubMed:34937913
MethodsX-ray diffraction
Resolution2.5 Å
Structure data

PDB-7jsd:
Hydroxylase homolog of BesD with Fe(II), alpha-ketoglutarate, and lysine
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-LYS:
LYSINE

ChemComp-AKG:
2-OXOGLUTARIC ACID

ChemComp-FE2:
Unknown entry

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

Source
  • streptomyces roseifaciens (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / Hydroxylase / non-heme iron

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