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-Structure paper
タイトル | The epitope arrangement on flavivirus particles contributes to Mab C10's extraordinary neutralization breadth across Zika and dengue viruses. |
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ジャーナル・号・ページ | Cell, Vol. 184, Issue 25, Page 6052-6066.e18, Year 2021 |
掲載日 | 2021年12月9日 |
著者 | Arvind Sharma / Xiaokang Zhang / Wanwisa Dejnirattisai / Xinghong Dai / Danyang Gong / Wiyada Wongwiwat / Stéphane Duquerroy / Alexander Rouvinski / Marie-Christine Vaney / Pablo Guardado-Calvo / Ahmed Haouz / Patrick England / Ren Sun / Z Hong Zhou / Juthathip Mongkolsapaya / Gavin R Screaton / Felix A Rey / |
PubMed 要旨 | The human monoclonal antibody C10 exhibits extraordinary cross-reactivity, potently neutralizing Zika virus (ZIKV) and the four serotypes of dengue virus (DENV1-DENV4). Here we describe a comparative ...The human monoclonal antibody C10 exhibits extraordinary cross-reactivity, potently neutralizing Zika virus (ZIKV) and the four serotypes of dengue virus (DENV1-DENV4). Here we describe a comparative structure-function analysis of C10 bound to the envelope (E) protein dimers of the five viruses it neutralizes. We demonstrate that the C10 Fab has high affinity for ZIKV and DENV1 but not for DENV2, DENV3, and DENV4. We further show that the C10 interaction with the latter viruses requires an E protein conformational landscape that limits binding to only one of the three independent epitopes per virion. This limited affinity is nevertheless counterbalanced by the particle's icosahedral organization, which allows two different dimers to be reached by both Fab arms of a C10 immunoglobulin. The epitopes' geometric distribution thus confers C10 its exceptional neutralization breadth. Our results highlight the importance not only of paratope/epitope complementarity but also the topological distribution for epitope-focused vaccine design. |
リンク | Cell / PubMed:34852239 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 2.1 - 3.6 Å |
構造データ | EMDB-30465, PDB-7cth: PDB-7a3n: PDB-7a3o: PDB-7a3p: PDB-7a3q: PDB-7a3r: PDB-7a3s: PDB-7a3t: PDB-7a3u: |
化合物 | ChemComp-CA: ChemComp-HOH: ChemComp-GOL: ChemComp-SO4: ChemComp-NAG: ChemComp-3CX: |
由来 |
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キーワード | VIRAL PROTEIN / FLAVIVIRUS / CLASS 2 FUSION PROTEIN / ANTIBODY / Fab / IMMUNOCOMPLEX / ZIKA / broadly neutralising antibody / DENGUE / F(ab')2 / VIRUS / Dengue virus / broadly neutralizing |