EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Intracellular nanoscale architecture as a master regulator of calcium carbonate crystallization in marine microalgae.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 118, Issue 46, Year 2021
掲載日	2021年11月16日
著者	Yuval Kadan / Fergus Tollervey / Neta Varsano / Julia Mahamid / Assaf Gal /
PubMed 要旨	Unicellular marine microalgae are responsible for one of the largest carbon sinks on Earth. This is in part due to intracellular formation of calcium carbonate scales termed coccoliths. ...Unicellular marine microalgae are responsible for one of the largest carbon sinks on Earth. This is in part due to intracellular formation of calcium carbonate scales termed coccoliths. Traditionally, the influence of changing environmental conditions on this process has been estimated using poorly constrained analogies to crystallization mechanisms in bulk solution, yielding ambiguous predictions. Here, we elucidated the intracellular nanoscale environment of coccolith formation in the model species using cryoelectron tomography. By visualizing cells at various stages of the crystallization process, we reconstructed a timeline of coccolith development. The three-dimensional data portray the native-state structural details of coccolith formation, uncovering the crystallization mechanism, and how it is spatially and temporally controlled. Most strikingly, the developing crystals are only tens of nanometers away from delimiting membranes, resulting in a highly confined volume for crystal growth. We calculate that the number of soluble ions that can be found in such a minute volume at any given time point is less than the number needed to allow the growth of a single atomic layer of the crystal and that the uptake of single protons can markedly affect nominal pH values. In such extreme confinement, the crystallization process is expected to depend primarily on the regulation of ion fluxes by the living cell, and nominal ion concentrations, such as pH, become the result, rather than a driver, of the crystallization process. These findings call for a new perspective on coccolith formation that does not rely exclusively on solution chemistry.
リンク	Proc Natl Acad Sci U S A / PubMed:34772804 / PubMed Central
手法	EM (トモグラフィー)
構造データ	EMDB-13755: P. carterae distal Golgi cisternae with immature base plate 手法: EM (トモグラフィー) EMDB-13756: P. carterae Golgi cisterna with forming coccolithosomes 手法: EM (トモグラフィー) EMDB-13757: P. carterae coccolith vesicle with mature base plate 手法: EM (トモグラフィー) EMDB-13758: P. carterae coccolith vesicle with nascent crystals 手法: EM (トモグラフィー) EMDB-13759: P. carterae coccolith vesicle with protococcolith ring 手法: EM (トモグラフィー) EMDB-13760: P. carterae coccolith vesicle with growing crystals 手法: EM (トモグラフィー) EMDB-13761: P. carterae coccolith vesicle with mature crystals 手法: EM (トモグラフィー)
由来	Chrysotila carterae (真核生物)