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TitleStructure of the hexameric fungal plasma membrane proton pump in its autoinhibited state.
Journal, issue, pagesSci Adv, Vol. 7, Issue 46, Page eabj5255, Year 2021
Publish dateNov 12, 2021
AuthorsSabine Heit / Maxwell M G Geurts / Bonnie J Murphy / Robin A Corey / Deryck J Mills / Werner Kühlbrandt / Maike Bublitz /
PubMed AbstractThe fungal plasma membrane H-ATPase Pma1 is a vital enzyme, generating a proton-motive force that drives the import of essential nutrients. Autoinhibited Pma1 hexamers in the plasma membrane of ...The fungal plasma membrane H-ATPase Pma1 is a vital enzyme, generating a proton-motive force that drives the import of essential nutrients. Autoinhibited Pma1 hexamers in the plasma membrane of starving fungi are activated by glucose signaling and subsequent phosphorylation of the autoinhibitory domain. As related P-type adenosine triphosphatases (ATPases) are not known to oligomerize, the physiological relevance of Pma1 hexamers remained unknown. We have determined the structure of hexameric Pma1 from by electron cryo-microscopy at 3.3-Å resolution, elucidating the molecular basis for hexamer formation and autoinhibition and providing a basis for structure-based drug development. Coarse-grained molecular dynamics simulations in a lipid bilayer suggest lipid-mediated contacts between monomers and a substantial protein-induced membrane deformation that could act as a proton-attracting funnel.
External linksSci Adv / PubMed:34757782 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 3.26 Å
Structure data

12638

7nxf

EMDB-12638, PDB-7nxf:
Structure of the fungal plasma membrane proton pump Pma1 in its auto-inhibited state - monomer unit
Method: EM (single particle) / Resolution: 3.1 Å

12644

7ny1

EMDB-12644, PDB-7ny1:
Structure of the fungal plasma membrane proton pump Pma1 in its auto-inhibited state - hexameric assembly
Method: EM (single particle) / Resolution: 3.26 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

Source
  • neurospora crassa (fungus)
KeywordsPROTON TRANSPORT / Membrane Protein / P-Type ATPase / proton-transporting ATPase

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