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Structure paper

TitleMolecular mechanism underlying substrate recognition of the peptide macrocyclase PsnB.
Journal, issue, pagesNat. Chem. Biol., Vol. 17, Page 1123-1131, Year 2021
Publish dateDec 29, 2020 (structure data deposition date)
AuthorsSong, I. / Kim, Y. / Yu, J. / Go, S.Y. / Lee, H.G. / Song, W.J. / Kim, S.
External linksNat. Chem. Biol. / PubMed:34475564
MethodsX-ray diffraction
Resolution2.98 - 3.56 Å
Structure data

PDB-7drm:
Structure of ATP-grasp ligase PsnB complexed with minimal precursor, Mg, and ADP
Method: X-RAY DIFFRACTION / Resolution: 3.28 Å

PDB-7drn:
Structure of ATP-grasp ligase PsnB complexed with precursor peptide PsnA2 and AMPPNP
Method: X-RAY DIFFRACTION / Resolution: 3.56 Å

PDB-7dro:
Structure of ATP-grasp ligase PsnB complexed with minimal precursor
Method: X-RAY DIFFRACTION / Resolution: 3.25 Å

PDB-7drp:
Structure of ATP-grasp ligase PsnB complexed with phosphomimetic variant of minimal precursor, Mg, and ADP
Method: X-RAY DIFFRACTION / Resolution: 2.98 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

Source
  • plesiocystis pacifica sir-1 (bacteria)
KeywordsLIGASE / ATP-grasp ligase / RiPP / graspetide / Omega ester bond containing peptide

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