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TitleInsights into the bilayer-mediated toppling mechanism of a folate-specific ECF transporter by cryo-EM.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 118, Issue 34, Year 2021
Publish dateAug 24, 2021
AuthorsChancievan Thangaratnarajah / Jan Rheinberger / Cristina Paulino / Dirk J Slotboom /
PubMed AbstractEnergy-coupling factor (ECF)-type transporters are small, asymmetric membrane protein complexes (∼115 kDa) that consist of a membrane-embedded, substrate-binding protein (S component) and a ...Energy-coupling factor (ECF)-type transporters are small, asymmetric membrane protein complexes (∼115 kDa) that consist of a membrane-embedded, substrate-binding protein (S component) and a tripartite ATP-hydrolyzing module (ECF module). They import micronutrients into bacterial cells and have been proposed to use a highly unusual transport mechanism, in which the substrate is dragged across the membrane by a toppling motion of the S component. However, it remains unclear how the lipid bilayer could accommodate such a movement. Here, we used cryogenic electron microscopy at 200 kV to determine structures of a folate-specific ECF transporter in lipid nanodiscs and detergent micelles at 2.7- and 3.4-Å resolution, respectively. The structures reveal an irregularly shaped bilayer environment around the membrane-embedded complex and suggest that toppling of the S component is facilitated by protein-induced membrane deformations. In this way, structural remodeling of the lipid bilayer environment is exploited to guide the transport process.
External linksProc Natl Acad Sci U S A / PubMed:34408021 / PubMed Central
MethodsEM (single particle)
Resolution2.7 - 3.4 Å
Structure data

12483

7nnt

EMDB-12483, PDB-7nnt:
Cryo-EM structure of the folate-specific ECF transporter complex in DDM micelles
Method: EM (single particle) / Resolution: 3.4 Å

12484

7nnu

EMDB-12484, PDB-7nnu:
Cryo-EM structure of the folate-specific ECF transporter complex in MSP2N2 lipid nanodiscs
Method: EM (single particle) / Resolution: 2.7 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • lactobacillus delbrueckii subsp. bulgaricus (strain atcc 11842 / dsm 20081 / jcm 1002 / nbrc 13953 / ncimb 11778) (bacteria)
KeywordsTRANSPORT PROTEIN / ABC Transporter / Type III ABC Transporter / ECF transporter complex / Folate transporter / Membrane protein

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