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Structure paper

TitleAn enzymatic activation of formaldehyde for nucleotide methylation.
Journal, issue, pagesNat Commun, Vol. 12, Page 4542-4542, Year 2021
Publish dateFeb 2, 2021 (structure data deposition date)
AuthorsBou-Nader, C. / Stull, F.W. / Pecqueur, L. / Simon, P. / Guerineau, V. / Royant, A. / Fontecave, M. / Lombard, M. / Palfey, B.A. / Hamdane, D.
External linksNat Commun / PubMed:34315871
MethodsX-ray diffraction
Resolution2 - 2.7 Å
Structure data

PDB-7ndw:
ThyX-FADH2 soaked with 20 mM Formaldehyde
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-7ndz:
ThyX reconstituted with N5-carbinolamine flavin
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-HUF:
[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3S,4S)-5-[5-methanoyl-7,8-dimethyl-2,4-bis(oxidanylidene)-1H-benzo[g]pteridin-10-yl]-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-PO4:
PHOSPHATE ION

ChemComp-FDA:
DIHYDROFLAVINE-ADENINE DINUCLEOTIDE

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-PGE:
TRIETHYLENE GLYCOL

Source
  • thermotoga maritima (bacteria)
KeywordsTRANSFERASE / FLAVIN-DEPENDENT THYMIDYLATE SYNTHASE / METHYLENETETRAHYDROFOLATE

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