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TitleMechanistic insight into bacterial entrapment by septin cage reconstitution.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 4511, Year 2021
Publish dateJul 23, 2021
AuthorsDamián Lobato-Márquez / Jingwei Xu / Gizem Özbaykal Güler / Adaobi Ojiakor / Martin Pilhofer / Serge Mostowy /
PubMed AbstractSeptins are cytoskeletal proteins that assemble into hetero-oligomeric complexes and sense micron-scale membrane curvature. During infection with Shigella flexneri, an invasive enteropathogen, ...Septins are cytoskeletal proteins that assemble into hetero-oligomeric complexes and sense micron-scale membrane curvature. During infection with Shigella flexneri, an invasive enteropathogen, septins restrict actin tail formation by entrapping bacteria in cage-like structures. Here, we reconstitute septin cages in vitro using purified recombinant septin complexes (SEPT2-SEPT6-SEPT7), and study how these recognize bacterial cells and assemble on their surface. We show that septin complexes recognize the pole of growing Shigella cells. An amphipathic helix domain in human SEPT6 enables septins to sense positively curved membranes and entrap bacterial cells. Shigella strains lacking lipopolysaccharide components are more efficiently entrapped in septin cages. Finally, cryo-electron tomography of in vitro cages reveals how septins assemble as filaments on the bacterial cell surface.
External linksNat Commun / PubMed:34301939 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-12562:
Cryo electron tomogram of Mycobacterium smegmatis entrapped in septin cages.
Method: EM (tomography)

EMDB-12565:
Cryo electron tomogram of Mycobacterium smegmatis
Method: EM (tomography)

EMDB-12571:
Cryo electron tomogram of Shigella flexneri entrapped in septin cages.
Method: EM (tomography)

EMDB-12578:
Cryo electron tomogram of Shigella flexneri
Method: EM (tomography)

EMDB-12579:
Cryo electron tomogram of Shigella flexneri drfaC mutant entrapped in septin cages.
Method: EM (tomography)

EMDB-12580:
Cryo electron tomogram of Shigella flexneri drfaC mutant
Method: EM (tomography)

Source
  • Mycolicibacterium smegmatis (bacteria)
  • Shigella flexneri (bacteria)

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