EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Three epitope-distinct human antibodies from RenMab mice neutralize SARS-CoV-2 and cooperatively minimize the escape of mutants.
ジャーナル・号・ページ	Cell Discov, Vol. 7, Issue 1, Page 53, Year 2021
掲載日	2021年7月20日
著者	Jianhui Nie / Jingshu Xie / Shuo Liu / Jiajing Wu / Chuan Liu / Jianhui Li / Yacui Liu / Meiyu Wang / Huizhen Zhao / Yabo Zhang / Jiawei Yao / Lei Chen / Yuelei Shen / Yi Yang / Hong-Wei Wang / Youchun Wang / Weijin Huang /
PubMed 要旨	Coronavirus disease 2019 (COVID-19), a pandemic disease caused by the newly emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused more than 3.8 million deaths to date. ...Coronavirus disease 2019 (COVID-19), a pandemic disease caused by the newly emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused more than 3.8 million deaths to date. Neutralizing antibodies are effective therapeutic measures. However, many naturally occurring mutations at the receptor-binding domain (RBD) have emerged, and some of them can evade existing neutralizing antibodies. Here, we utilized RenMab, a novel mouse carrying the entire human antibody variable region, for neutralizing antibody discovery. We obtained several potent RBD-blocking antibodies and categorized them into four distinct groups by epitope mapping. We determined the involved residues of the epitope of three representative antibodies by cryo-electron microscopy (Cryo-EM) studies. Moreover, we performed neutralizing experiments with 50 variant strains with single or combined mutations and found that the mixing of three epitope-distinct antibodies almost eliminated the mutant escape. Our study provides a sound basis for the rational design of fully human antibody cocktails against SARS-CoV-2 and pre-emergent coronaviral threats.
リンク	Cell Discov / PubMed:34285195 / PubMed Central
手法	EM (単粒子)
解像度	3.3 - 4.2 Å
構造データ	EMDB-30976: SARS-CoV-2 spike in complex with the Ab4 neutralizing antibody (State 1) 手法: EM (単粒子) / 解像度: 3.4 Å 30977 7e39 EMDB-30977, PDB-7e39: SARS-CoV-2 spike in complex with the Ab4 neutralizing antibody (State 3) 手法: EM (単粒子) / 解像度: 3.7 Å 30978 7e3b EMDB-30978, PDB-7e3b: SARS-Cov-2 spike in complex with the Ab5 neutralizing antibody (focused refinement on Fab-RBD) 手法: EM (単粒子) / 解像度: 4.2 Å 30979 7e3c EMDB-30979, PDB-7e3c: SARS-CoV-2 spike in complex with the Ab1 neutralizing antibody (focused refinement on Fab-RBD) 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-30980: SARS-CoV-2 spike in complex with the Ab5 neutralizing antibody Fab (consensus map) 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-30981: SARS-CoV-2 spike in complex with the Ab1 neutralizing antibody (consensus map) 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN/IMMUNE SYSTEM (ウイルス性) / Spike / SARS-CoV-2 (SARSコロナウイルス2) / Antibody (抗体) / VIRAL PROTEIN (ウイルスタンパク質) / VIRAL PROTEIN-IMMUNE SYSTEM complex (ウイルス性)