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TitleShotgun EM of mycobacterial protein complexes during stationary phase stress.
Journal, issue, pagesCurr Res Struct Biol, Vol. 2, Page 204-212, Year 2020
Publish dateSep 22, 2020
AuthorsAngela M Kirykowicz / Jeremy D Woodward /
PubMed AbstractThere is little structural information about the protein complexes conferring resistance in to anti-microbial oxygen and nitrogen radicals in the phagolysosome. Here, we expose the model ...There is little structural information about the protein complexes conferring resistance in to anti-microbial oxygen and nitrogen radicals in the phagolysosome. Here, we expose the model Mycobacterium, to simulated oxidative-stress conditions and apply a shotgun EM method for the structural detection of the resulting protein assemblies. We identified: glutamine synthetase I, essential for virulence; bacterioferritin A, critical for iron regulation; aspartyl aminopeptidase M18, a protease; and encapsulin, which produces a cage-like structure to enclose cargo proteins. After further investigation, we found that encapsulin carries dye-decolourising peroxidase, a protein antioxidant, as its primary cargo under the conditions tested.
External linksCurr Res Struct Biol / PubMed:34235480 / PubMed Central
MethodsEM (single particle)
Resolution16.0 - 27.0 Å
Structure data

EMDB-11595:
Shotgun EM of Mycobacterial protein complexes during stationary phase stress.
Method: EM (single particle) / Resolution: 26.0 Å

EMDB-11596:
Shotgun EM of Mycobacterial protein complexes during stationary phase stress
Method: EM (single particle) / Resolution: 16.0 Å

EMDB-11597:
Shotgun EM of Mycobacterial protein complexes during stationary phase stress.
Method: EM (single particle) / Resolution: 18.0 Å

EMDB-11598:
Shotgun EM of Mycobacterial protein complexes during stationary phase stress.
Method: EM (single particle) / Resolution: 27.0 Å

EMDB-11599:
Shotgun EM of Mycobacterial protein complexes during stationary phase stress
Method: EM (single particle) / Resolution: 19.0 Å

Source
  • Mycolicibacterium smegmatis MC2 155 (bacteria)

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