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-Structure paper
| タイトル | Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063. |
|---|---|
| ジャーナル・号・ページ | Nat Plants, Vol. 7, Issue 8, Page 1132-1142, Year 2021 |
| 掲載日 | 2021年7月5日 |
著者 | Yanan Xiao / Guoqiang Huang / Xin You / Qingjun Zhu / Wenda Wang / Tingyun Kuang / Guangye Han / Sen-Fang Sui / Jian-Ren Shen / ![]() |
| PubMed 要旨 | Photosystem II (PSII) is a multisubunit pigment-protein complex and catalyses light-induced water oxidation, leading to the conversion of light energy into chemical energy and the release of dioxygen. ...Photosystem II (PSII) is a multisubunit pigment-protein complex and catalyses light-induced water oxidation, leading to the conversion of light energy into chemical energy and the release of dioxygen. We analysed the structures of two Psb28-bound PSII intermediates, Psb28-RC47 and Psb28-PSII, purified from a psbV-deletion strain of the thermophilic cyanobacterium Thermosynechococcus vulcanus, using cryo-electron microscopy. Both Psb28-RC47 and Psb28-PSII bind one Psb28, one Tsl0063 and an unknown subunit. Psb28 is located at the cytoplasmic surface of PSII and interacts with D1, D2 and CP47, whereas Tsl0063 is a transmembrane subunit and binds at the side of CP47/PsbH. Substantial structural perturbations are observed at the acceptor side, which result in conformational changes of the quinone (Q) and non-haem iron binding sites and thus may protect PSII from photodamage during assembly. These results provide a solid structural basis for understanding the assembly process of native PSII. |
リンク | Nat Plants / PubMed:34226692 |
| 手法 | EM (単粒子) |
| 解像度 | 3.14 Å |
| 構造データ | EMDB-30902, PDB-7dxa: EMDB-30909, PDB-7dxh: |
| 化合物 | ![]() ChemComp-SQD: ![]() ChemComp-CLA: ![]() ChemComp-PHO: ![]() ChemComp-BCR: ![]() ChemComp-CL: ![]() ChemComp-MGE: ![]()
ChemComp-UNL: ![]() ChemComp-LMT: ![]() ChemComp-FE2: ![]() ChemComp-PQ9: ![]() ChemComp-HTG: ![]() ChemComp-DGD: ![]() ChemComp-HEM: |
| 由来 |
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キーワード | PLANT PROTEIN / Photosystem II; Cryo-EM; assembly; repair; Psb28; Tsl0063 |
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thermosynechococcus vulcanus (バクテリア)
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