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TitleStructure and substrate recognition by the Ruminococcus bromii amylosome pullulanases.
Journal, issue, pagesJ. Struct. Biol., Vol. 213, Page 107765-107765, Year 2021
Publish dateFeb 18, 2021 (structure data deposition date)
AuthorsCockburn, D.W. / Kibler, R. / Brown, H.A. / Duvall, R. / Morais, S. / Bayer, E. / Koropatkin, N.M.
External linksJ. Struct. Biol. / PubMed:34186214
MethodsX-ray diffraction
Resolution1.76 - 2.42 Å
Structure data

PDB-7lsa:
Ruminococcus bromii Amy12 with maltoheptaose
Method: X-RAY DIFFRACTION / Resolution: 1.76 Å

PDB-7lsr:
Ruminococcus bromii Amy12-D392A with maltoheptaose
Method: X-RAY DIFFRACTION / Resolution: 2.42 Å

PDB-7lst:
Ruminococcus bromii Amy12-D392A with 63-a-D-glucosyl-maltotriosyl-maltotriose
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-7lsu:
Ruminococcus bromii Amy12-D392A with 63-a-D-glucosyl-maltotriose
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-ACT:
ACETATE ION

ChemComp-NA:
Unknown entry

Source
  • ruminococcus bromii (bacteria)
KeywordsHYDROLASE / GH13 / pullulanase / CBM48 / complex

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