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TitleA Novel N-terminal Region to Chromodomain in CHD7 is Required for the Efficient Remodeling Activity.
Journal, issue, pagesJ Mol Biol, Vol. 433, Issue 18, Page 167114, Year 2021
Publish dateSep 3, 2021
AuthorsEunhye Lee / Chanshin Kang / Pasi Purhonen / Hans Hebert / Karim Bouazoune / Sungchul Hohng / Ji-Joon Song /
PubMed AbstractChromodomain-Helicase DNA binding protein 7 (CHD7) is an ATP dependent chromatin remodeler involved in maintaining open chromatin structure. Mutations of CHD7 gene causes multiple developmental ...Chromodomain-Helicase DNA binding protein 7 (CHD7) is an ATP dependent chromatin remodeler involved in maintaining open chromatin structure. Mutations of CHD7 gene causes multiple developmental disorders, notably CHARGE syndrome. However, there is not much known about the molecular mechanism by which CHD7 remodels nucleosomes. Here, we performed biochemical and biophysical analysis on CHD7 chromatin remodeler and uncover that N-terminal to the Chromodomain (N-CRD) interacts with nucleosome and contains a high conserved arginine stretch, which is reminiscent of arginine anchor. Importantly, this region is required for efficient ATPase stimulation and nucleosome remodeling activity of CHD7. Furthermore, smFRET analysis shows the mutations in the N-CRD causes the defects in remodeling activity. Collectively, our results uncover the functional importance of a previously unidentified N-terminal region in CHD7 and implicate that the multiple domains in chromatin remodelers are involved in regulating their activities.
External linksJ Mol Biol / PubMed:34161779
MethodsEM (single particle)
Resolution7.14 - 7.25 Å
Structure data

EMDB-30298:
CryoEM structure of CHD7(N-CRD) bound to the nucleosome
Method: EM (single particle) / Resolution: 7.25 Å

EMDB-30630:
CHD7 N-CRD and nucleosome(+40bp) complex
Method: EM (single particle) / Resolution: 7.14 Å

Source
  • Homo sapiens (human)
  • Xenopus laevis (African clawed frog)
  • synthetic construct (others)

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