EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling.
ジャーナル・号・ページ	Nat Commun, Vol. 12, Issue 1, Page 3672, Year 2021
掲載日	2021年6月16日
著者	Hauke S Hillen / Elena Lavdovskaia / Franziska Nadler / Elisa Hanitsch / Andreas Linden / Katherine E Bohnsack / Henning Urlaub / Ricarda Richter-Dennerlein /
PubMed 要旨	Ribosome biogenesis requires auxiliary factors to promote folding and assembly of ribosomal proteins and RNA. Particularly, maturation of the peptidyl transferase center (PTC) is mediated by ...Ribosome biogenesis requires auxiliary factors to promote folding and assembly of ribosomal proteins and RNA. Particularly, maturation of the peptidyl transferase center (PTC) is mediated by conserved GTPases, but the molecular basis is poorly understood. Here, we define the mechanism of GTPase-driven maturation of the human mitochondrial large ribosomal subunit (mtLSU) using endogenous complex purification, in vitro reconstitution and cryo-EM. Structures of transient native mtLSU assembly intermediates that accumulate in GTPBP6-deficient cells reveal how the biogenesis factors GTPBP5, MTERF4 and NSUN4 facilitate PTC folding. Addition of recombinant GTPBP6 reconstitutes late mtLSU biogenesis in vitro and shows that GTPBP6 triggers a molecular switch and progression to a near-mature PTC state. Additionally, cryo-EM analysis of GTPBP6-treated mature mitochondrial ribosomes reveals the structural basis for the dual-role of GTPBP6 in ribosome biogenesis and recycling. Together, these results provide a framework for understanding step-wise PTC folding as a critical conserved quality control checkpoint.
リンク	Nat Commun / PubMed:34135319 / PubMed Central
手法	EM (単粒子)
解像度	2.2 - 2.9 Å
構造データ	12865 7of0 EMDB-12865, PDB-7of0: Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 (dataset1). 手法: EM (単粒子) / 解像度: 2.2 Å 12867 7of2 EMDB-12867, PDB-7of2: Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with GTPBP6. 手法: EM (単粒子) / 解像度: 2.7 Å 12868 7of3 EMDB-12868, PDB-7of3: Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 (dataset2). 手法: EM (単粒子) / 解像度: 2.7 Å 12869 7of4 EMDB-12869, PDB-7of4: Structure of mature human mitochondrial ribosome large subunit in complex with GTPBP6 (PTC conformation 1). 手法: EM (単粒子) / 解像度: 2.7 Å 12870 7of5 EMDB-12870, PDB-7of5: Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 and GTPBP5 (dataset2). 手法: EM (単粒子) / 解像度: 2.9 Å 12871 7of6 EMDB-12871, PDB-7of6: Structure of mature human mitochondrial ribosome large subunit in complex with GTPBP6 (PTC conformation 2). 手法: EM (単粒子) / 解像度: 2.6 Å 12872 7of7 EMDB-12872, PDB-7of7: Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 and GTPBP5 (dataset1). 手法: EM (単粒子) / 解像度: 2.5 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子*YM
由来	homo sapiens (ヒト)
キーワード	RIBOSOME / Mitochondria / Biogenesis / GTPase / NSUN4 / MTERF4