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-Structure paper
タイトル | Cryo-EM structure of TFIIH/Rad4-Rad23-Rad33 in damaged DNA opening in nucleotide excision repair. |
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ジャーナル・号・ページ | Nat Commun, Vol. 12, Issue 1, Page 3338, Year 2021 |
掲載日 | 2021年6月7日 |
![]() | Trevor van Eeuwen / Yoonjung Shim / Hee Jong Kim / Tingting Zhao / Shrabani Basu / Benjamin A Garcia / Craig D Kaplan / Jung-Hyun Min / Kenji Murakami / ![]() |
PubMed 要旨 | The versatile nucleotide excision repair (NER) pathway initiates as the XPC-RAD23B-CETN2 complex first recognizes DNA lesions from the genomic DNA and recruits the general transcription factor ...The versatile nucleotide excision repair (NER) pathway initiates as the XPC-RAD23B-CETN2 complex first recognizes DNA lesions from the genomic DNA and recruits the general transcription factor complex, TFIIH, for subsequent lesion verification. Here, we present a cryo-EM structure of an NER initiation complex containing Rad4-Rad23-Rad33 (yeast homologue of XPC-RAD23B-CETN2) and 7-subunit coreTFIIH assembled on a carcinogen-DNA adduct lesion at 3.9-9.2 Å resolution. A ~30-bp DNA duplex could be mapped as it straddles between Rad4 and the Ssl2 (XPB) subunit of TFIIH on the 3' and 5' side of the lesion, respectively. The simultaneous binding with Rad4 and TFIIH was permitted by an unwinding of DNA at the lesion. Translocation coupled with torque generation by Ssl2 and Rad4 would extend the DNA unwinding at the lesion and deliver the damaged strand to Rad3 (XPD) in an open form suitable for subsequent lesion scanning and verification. |
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手法 | EM (単粒子) |
解像度 | 3.9 - 9.25 Å |
構造データ | EMDB-22576: TFIIH/Rad4-23-33 Complex in NER focused on Rad4-33 EMDB-22587, PDB-7k01: EMDB-22588, PDB-7k04: |
化合物 | ![]() ChemComp-ZN: ![]() ChemComp-SF4: ![]() ChemComp-CA: |
由来 |
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![]() | NUCLEAR PROTEIN/Transferase / TFIIH / Rad4 / Rad4/23 / XPC / NER / Nucleotide Excision Repair / GG-NER / NUCLEAR PROTEIN / NUCLEAR PROTEIN-Transferase complex / NUCLEAR PROTEIN/DNA / NUCLEAR PROTEIN-DNA complex |