EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for broad coronavirus neutralization.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 28, Issue 6, Page 478-486, Year 2021
掲載日	2021年5月12日
著者	Maximilian M Sauer / M Alejandra Tortorici / Young-Jun Park / Alexandra C Walls / Leah Homad / Oliver J Acton / John E Bowen / Chunyan Wang / Xiaoli Xiong / Willem de van der Schueren / Joel Quispe / Benjamin G Hoffstrom / Berend-Jan Bosch / Andrew T McGuire / David Veesler /
PubMed 要旨	Three highly pathogenic β-coronaviruses have crossed the animal-to-human species barrier in the past two decades: SARS-CoV, MERS-CoV and SARS-CoV-2. To evaluate the possibility of identifying ...Three highly pathogenic β-coronaviruses have crossed the animal-to-human species barrier in the past two decades: SARS-CoV, MERS-CoV and SARS-CoV-2. To evaluate the possibility of identifying antibodies with broad neutralizing activity, we isolated a monoclonal antibody, termed B6, that cross-reacts with eight β-coronavirus spike glycoproteins, including all five human-infecting β-coronaviruses. B6 broadly neutralizes entry of pseudotyped viruses from lineages A and C, but not from lineage B, and the latter includes SARS-CoV and SARS-CoV-2. Cryo-EM, X-ray crystallography and membrane fusion assays reveal that B6 binds to a conserved cryptic epitope located in the fusion machinery. The data indicate that antibody binding sterically interferes with the spike conformational changes leading to membrane fusion. Our data provide a structural framework explaining B6 cross-reactivity with β-coronaviruses from three lineages, along with a proof of concept for antibody-mediated broad coronavirus neutralization elicited through vaccination. This study unveils an unexpected target for next-generation structure-guided design of a pan-β-coronavirus vaccine.
リンク	Nat Struct Mol Biol / PubMed:33981021
手法	EM (単粒子) / X線回折
解像度	1.4 - 4.7 Å
構造データ	EMDB-23672: Structural basis for broad coronavirus neutralization 手法: EM (単粒子) / 解像度: 4.7 Å 23674 7m5e EMDB-23674, PDB-7m5e: MERS-CoV S bound to the broadly neutralizing B6 Fab fragment (C3 refinement) 手法: EM (単粒子) / 解像度: 2.5 Å PDB-7m51: B6 Fab fragment bound to the OC43 spike stem helix peptide 手法: X-RAY DIFFRACTION / 解像度: 1.8 Å PDB-7m52: B6 Fab fragment bound to the HKU4 spike stem helix peptide 手法: X-RAY DIFFRACTION / 解像度: 1.5 Å PDB-7m53: B6 Fab fragment bound to the SARS-CoV/SARS-CoV-2 spike stem helix peptide 手法: X-RAY DIFFRACTION / 解像度: 1.4 Å PDB-7m55: B6 Fab fragment bound to the MERS-CoV spike stem helix peptide 手法: X-RAY DIFFRACTION / 解像度: 1.4 Å
化合物	ChemComp-GOL: GLYCEROL / グリセロ-ル / グリセリン ChemComp-HOH: WATER / 水 ChemComp-FOL: FOLIC ACID / 葉酸 / 葉酸 ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-SIA: N-acetyl-alpha-neuraminic acid / N-アセチル-α-ノイラミン酸 / シアル酸
由来	middle east respiratory syndrome-related coronavirus (MERSコロナウイルス) mus musculus (ハツカネズミ) human coronavirus oc43 (ヒトコロナウイルス OC43) bat coronavirus hku4 (ウイルス) severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2)
キーワード	ANTIVIRAL PROTEIN / IMMUNE SYSTEM (免疫系) / Broadly neutralizing antibody (中和抗体) / Structural genomics (構造ゲノミクス) / SSGCID / Seattle Structural Genomics Center for Infectious Disease / Center for Structural Genomics of Infectious Diseases / CSGID / VIRAL PROTEIN (ウイルスタンパク質) / MERS-CoV (MERSコロナウイルス) / coronaviruses / antibody (抗体)