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TitleCryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export.
Journal, issue, pagesmBio, Vol. 12, Issue 2, Year 2021
Publish dateApr 5, 2021
AuthorsMitchell A Moseng / Meinan Lyu / Tanadet Pipatpolkai / Przemyslaw Glaza / Corey C Emerson / Phoebe L Stewart / Phillip J Stansfeld / Edward W Yu /
PubMed AbstractGram-negative bacteria utilize the resistance-nodulation-cell division (RND) superfamily of efflux pumps to expel a variety of toxic compounds from the cell. The CusA membrane protein, which ...Gram-negative bacteria utilize the resistance-nodulation-cell division (RND) superfamily of efflux pumps to expel a variety of toxic compounds from the cell. The CusA membrane protein, which recognizes and extrudes biocidal Cu(I) and Ag(I) ions, belongs to the heavy-metal efflux (HME) subfamily of RND efflux pumps. We here report four structures of the trimeric CusA heavy-metal efflux pump in the presence of Cu(I) using single-particle cryo-electron microscopy (cryo-EM). We discover that different CusA protomers within the trimer are able to bind Cu(I) ions simultaneously. Our structural data combined with molecular dynamics (MD) simulations allow us to propose a mechanism for ion transport where each CusA protomer functions independently within the trimer. The bacterial RND superfamily of efflux pumps mediate resistance to a variety of biocides, including Cu(I) and Ag(I) ions. Here we report four cryo-EM structures of the trimeric CusA pump in the presence of Cu(I). Combined with MD simulations, our data indicate that each CusA protomer within the trimer recognizes and extrudes Cu(I) independently.
External linksmBio / PubMed:33820823 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 3.4 Å
Structure data

22843

7kf5

EMDB-22843, PDB-7kf5:
Cryo-electron microscopy structure of the heavy metal efflux pump CusA in the symmetric closed state
Method: EM (single particle) / Resolution: 3.2 Å

22844

7kf6

EMDB-22844, PDB-7kf6:
Cryo-electron microscopy structure of the heavy metal efflux pump CusA in a homogeneous binding copper(1) state
Method: EM (single particle) / Resolution: 3.4 Å

22845

7kf7

EMDB-22845, PDB-7kf7:
Cryo-electron microscopy structure of the heavy metal efflux pump CusA in a heterogeneous 1 open and 2 closed protomer conformation
Method: EM (single particle) / Resolution: 2.8 Å

22846

7kf8

EMDB-22846, PDB-7kf8:
Cryo-electron microscopy structure of the heavy metal efflux pump CusA in a heterogeneous 2 open and 1 closed protomer conformation
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-CU1:
COPPER (I) ION / Copper

Source
  • escherichia coli (E. coli)
KeywordsMEMBRANE PROTEIN / TRANSPORT PROTEIN / efflux / pump / heavy metal. copper / silver / closed / open / transport / MEMBRANE PROTEIN/ TRANSPORT PROTEIN / transporter / MEMBRANE PROTEIN- TRANSPORT PROTEIN complex

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