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TitleCryo-EM structures of human coagulation factors V and Va.
Journal, issue, pagesBlood, Vol. 137, Issue 22, Page 3137-3144, Year 2021
Publish dateJun 3, 2021
AuthorsEliza A Ruben / Michael J Rau / James A J Fitzpatrick / Enrico Di Cera /
PubMed AbstractCoagulation factor V (fV) is the precursor of fVa, which, together with fXa, Ca2+, and phospholipids, defines the prothrombinase complex and activates prothrombin in the penultimate step of the ...Coagulation factor V (fV) is the precursor of fVa, which, together with fXa, Ca2+, and phospholipids, defines the prothrombinase complex and activates prothrombin in the penultimate step of the coagulation cascade. We solved the cryogenic electron microscopy (cryo-EM) structures of human fV and fVa at atomic (3.3 Å) and near-atomic (4.4 Å) resolution, respectively. The structure of fV reveals the entire A1-A2-B-A3-C1-C2 assembly, but with a surprisingly disordered B domain. The C1 and C2 domains provide a platform for interaction with phospholipid membranes and support the A1 and A3 domains, with the A2 domain sitting on top of them. The B domain is highly dynamic and visible only for short segments connecting to the A2 and A3 domains. The A2 domain reveals all sites of proteolytic processing by thrombin and activated protein C, a partially buried epitope for binding fXa, and fully exposed epitopes for binding activated protein C and prothrombin. Removal of the B domain and activation to fVa exposes the sites of cleavage by activated protein C at R306 and R506 and produces increased disorder in the A1-A2-A3-C1-C2 assembly, especially in the C-terminal acidic portion of the A2 domain that is responsible for prothrombin binding. Ordering of this region and full exposure of the fXa epitope emerge as necessary steps in the assembly of the prothrombin-prothrombinase complex. These structures offer molecular context for the function of fV and fVa and pioneer the analysis of coagulation factors by cryo-EM.
External linksBlood / PubMed:33684942 / PubMed Central
MethodsEM (single particle)
Resolution3.3 - 4.4 Å
Structure data

23048

7kve

EMDB-23048, PDB-7kve:
Cryo-EM structure of human Factor V at 3.3 Angstrom resolution
Method: EM (single particle) / Resolution: 3.3 Å

23049

7kvf

EMDB-23049, PDB-7kvf:
Cryo-EM structure of human Factor V at 3.6 Angstrom resolution
Method: EM (single particle) / Resolution: 3.6 Å

23067

7kxy

EMDB-23067, PDB-7kxy:
Cryo-EM structure of human Factor Va at 4.4 Angstrom resolution
Method: EM (single particle) / Resolution: 4.4 Å

Source
  • homo sapiens (human)
  • Human (human)
KeywordsBLOOD CLOTTING / human Factor V / Factor Va

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