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Structure paper

TitleEngineering subtilisin proteases that specifically degrade active RAS.
Journal, issue, pagesCommun Biol, Vol. 4, Page 299-299, Year 2021
Publish dateSep 9, 2019 (structure data deposition date)
AuthorsChen, Y. / Toth, E.A. / Ruan, B. / Choi, E.J. / Simmerman, R. / He, Y. / Wang, R. / Godoy-Ruiz, R. / King, H. / Custer, G. ...Chen, Y. / Toth, E.A. / Ruan, B. / Choi, E.J. / Simmerman, R. / He, Y. / Wang, R. / Godoy-Ruiz, R. / King, H. / Custer, G. / Travis Gallagher, D. / Rozak, D.A. / Solomon, M. / Muro, S. / Weber, D.J. / Orban, J. / Fuerst, T.R. / Bryan, P.N.
External linksCommun Biol / PubMed:33674772
MethodsX-ray diffraction
Resolution1.2 - 1.7 Å
Structure data

PDB-6u9l:
Imidazole-triggered RAS-specific subtilisin SUBT_BACAM
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-6uai:
Imidazole-triggered RAS-specific subtilisin SUBT_BACAM complexed with YSAM peptide
Method: X-RAY DIFFRACTION / Resolution: 1.2 Å

PDB-6uao:
Imidazole-triggered RAS-specific subtilisin SUBT_BACAM complexed with the peptide EEYSAM
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

PDB-6ube:
Azide-triggered subtilisin SUBT_BACAM complexed with the peptide LFRAL
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

Chemicals

ChemComp-K:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-SCN:
THIOCYANATE ION

ChemComp-HOH:
WATER

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-AZI:
AZIDE ION

Source
  • bacillus amyloliquefaciens (bacteria)
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsHYDROLASE / engineered protease

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