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Structure paper

TitleStructural basis for differential recognition of phosphohistidine-containing peptides by 1-pHis and 3-pHis monoclonal antibodies.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 118, Year 2021
Publish dateMay 19, 2020 (structure data deposition date)
AuthorsKalagiri, R. / Stanfield, R.L. / Meisenhelder, J. / La Clair, J.J. / Fuhs, S.R. / Wilson, I.A. / Hunter, T.
External linksProc. Natl. Acad. Sci. USA / PubMed:33547238
MethodsX-ray diffraction
Resolution1.641 - 2.34 Å
Structure data

PDB-6x1s:
Structure of pHis Fab (SC1-1) in complex with pHis mimetic peptide
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-6x1t:
Structure of pHis Fab (SC50-3) in complex with pHis mimetic peptide
Method: X-RAY DIFFRACTION / Resolution: 2.34 Å

PDB-6x1u:
Structure of pHis Fab (SC39-4) in complex with pHis mimetic peptide
Method: X-RAY DIFFRACTION / Resolution: 1.641 Å

PDB-6x1v:
Structure of pHis Fab (SC44-8) in complex with pHis mimetic peptide
Method: X-RAY DIFFRACTION / Resolution: 2.11 Å

PDB-6x1w:
Structure of pHis Fab (SC56-2) in complex with pHis mimetic peptide
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-ACT:
ACETATE ION

Source
  • oryctolagus cuniculus (rabbit)
  • synthetic construct (others)
KeywordsIMMUNE SYSTEM / Anti-phosphohistidine antibody / post-translational modification

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