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Structure paper

TitleGTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation.
Journal, issue, pagesJ. Cell Biol., Vol. 220, Year 2021
Publish dateNov 22, 2019 (structure data deposition date)
AuthorsAyukawa, R. / Iwata, S. / Imai, H. / Kamimura, S. / Hayashi, M. / Ngo, K.X. / Minoura, I. / Uchimura, S. / Makino, T. / Shirouzu, M. ...Ayukawa, R. / Iwata, S. / Imai, H. / Kamimura, S. / Hayashi, M. / Ngo, K.X. / Minoura, I. / Uchimura, S. / Makino, T. / Shirouzu, M. / Shigematsu, H. / Sekimoto, K. / Gigant, B. / Muto, E.
External linksJ. Cell Biol. / PubMed:33544140
MethodsX-ray diffraction
Resolution2.197 - 3.571 Å
Structure data

PDB-6tis:
DROSOPHILA GDP-TUBULIN
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-6tiu:
DROSOPHILA GTP-TUBULIN Y222F MUTANT
Method: X-RAY DIFFRACTION / Resolution: 3.571 Å

PDB-6tiy:
DROSOPHILA GMPCPP-TUBULIN
Method: X-RAY DIFFRACTION / Resolution: 2.293 Å

PDB-6tiz:
DROSOPHILA GDP-TUBULIN Y222F MUTANT
Method: X-RAY DIFFRACTION / Resolution: 2.197 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-HOH:
WATER

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

Source
  • drosophila melanogaster (fruit fly)
  • rattus norvegicus (Norway rat)
KeywordsCELL CYCLE / MICROTUBULE / MICROTUBULE DYNAMICS / MICROTULE NUCLEATION

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