EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Atomic structures of respiratory complex III, complex IV, and supercomplex III-IV from vascular plants.
ジャーナル・号・ページ	Elife, Vol. 10, Year 2021
掲載日	2021年1月19日
著者	Maria Maldonado / Fei Guo / James A Letts /
PubMed 要旨	Mitochondrial complex III (CIII) and complex IV (CIV), which can associate into a higher-order supercomplex (SC III+IV), play key roles in respiration. However, structures of these plant complexes ...Mitochondrial complex III (CIII) and complex IV (CIV), which can associate into a higher-order supercomplex (SC III+IV), play key roles in respiration. However, structures of these plant complexes remain unknown. We present atomic models of CIII, CIV, and SC III+IV from determined by single-particle cryoEM. The structures reveal plant-specific differences in the MPP domain of CIII and define the subunit composition of CIV. Conformational heterogeneity analysis of CIII revealed long-range, coordinated movements across the complex, as well as the motion of CIII's iron-sulfur head domain. The CIV structure suggests that, in plants, proton translocation does not occur via the H channel. The supercomplex interface differs significantly from that in yeast and bacteria in its interacting subunits, angle of approach and limited interactions in the mitochondrial matrix. These structures challenge long-standing assumptions about the plant complexes and generate new mechanistic hypotheses.
リンク	Elife / PubMed:33463523 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 3.8 Å
構造データ	22445 7jrg EMDB-22445, PDB-7jrg: Plant Mitochondrial complex III2 from Vigna radiata 手法: EM (単粒子) / 解像度: 3.2 Å 22447 7jro EMDB-22447, PDB-7jro: Plant Mitochondrial complex IV from Vigna radiata 手法: EM (単粒子) / 解像度: 3.8 Å 22448 7jrp EMDB-22448: Plant Mitochondrial complex SC III2+IV from Vigna radiata composite map PDB-7jrp: Plant Mitochondrial complex SC III2+IV from Vigna radiata 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-22449: Plant mitochondrial supercomplex III2+IV from Vigna radiata 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-22450: Plant mitochondrial complex III2 from Vigna radiata supercomplex III2+IV, focused refinement. 手法: EM (単粒子) / 解像度: 3.7 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-CDL: CARDIOLIPIN / リン脂質YM ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM ChemComp-HEC: HEME C ChemComp-HEA: HEME-A ChemComp-CU: COPPER (II) ION ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CUA: DINUCLEAR COPPER ION ChemComp-LYS*: LYSINE / 2-アンモニオ-2-デアミノ-L-リシン
由来	vigna radiata (リョクトウ) Mung bean (リョクトウ) vigna radiata var. radiata (リョクトウ)
キーワード	ELECTRON TRANSPORT / mitochondria / respiration / bioenergetics / plants / OXIDOREDUCTASE